Coherent Raman Scattering Microscopy

Coherent Raman Scattering Microscopy
Author: Ji-Xin Cheng
Publisher: CRC Press
Total Pages: 603
Release: 2016-04-19
Genre: Science
ISBN: 1439867666

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The First Book on CRS MicroscopyCompared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Sc

Stimulated Raman Scattering Microscopy

Stimulated Raman Scattering Microscopy
Author: Ji-Xin Cheng
Publisher: Elsevier
Total Pages: 612
Release: 2021-12-04
Genre: Science
ISBN: 0323903371

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Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics

Deep Tissue Insights

Deep Tissue Insights
Author: Anton
Publisher: Tredition Gmbh
Total Pages: 0
Release: 2024-06-25
Genre: Science
ISBN: 9783384271488

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Light interacts with matter in various ways. CRS microscopy relies on a specific type of light-matter interaction known as Raman scattering. When light impinges on a molecule, it can inelastically scatter, meaning the molecule absorbs some energy and re-emits light at a slightly different frequency. This shift in frequency, known as the Raman shift, is unique to each molecule and acts as its spectral fingerprint. CRS microscopy leverages this principle to generate detailed chemical maps of a sample. By focusing a laser beam onto the sample and analyzing the scattered light, researchers can identify the specific molecules present based on their characteristic Raman shifts. Unlike traditional Raman spectroscopy, CRS microscopy employs coherent light sources, allowing for enhanced signal-to-noise ratio and deeper tissue penetration.

Molecules and Radiation

Molecules and Radiation
Author: Jeffrey I. Steinfeld
Publisher: Courier Corporation
Total Pages: 514
Release: 2012-11-09
Genre: Science
ISBN: 0486137546

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This unified treatment introduces upper-level undergraduates and graduate students to the concepts and methods of modern molecular spectroscopy and their applications to quantum electronics, lasers, and related optical phenomena. Starting with a review of the prerequisite quantum mechanical background, the text examines atomic spectra and diatomic molecules, including the rotation and vibration of diatomic molecules and their electronic spectra. A discussion of rudimentary group theory advances to considerations of the rotational spectra of polyatomic molecules and their vibrational and electronic spectra; molecular beams, masers, and lasers; and a variety of forms of spectroscopy, including optical resonance spectroscopy, coherent transient spectroscopy, multiple-photon spectroscopy, and spectroscopy beyond molecular constants. The text concludes with a series of useful appendixes.

Confocal Raman Microscopy

Confocal Raman Microscopy
Author: Jan Toporski
Publisher: Springer
Total Pages: 603
Release: 2018-03-01
Genre: Science
ISBN: 3319753800

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This second edition provides a cutting-edge overview of physical, technical and scientific aspects related to the widely used analytical method of confocal Raman microscopy. The book includes expanded background information and adds insights into how confocal Raman microscopy, especially 3D Raman imaging, can be integrated with other methods to produce a variety of correlative microscopy combinations. The benefits are then demonstrated and supported by numerous examples from the fields of materials science, 2D materials, the life sciences, pharmaceutical research and development, as well as the geosciences.

Coherent Raman Spectroscopy

Coherent Raman Spectroscopy
Author: Gerd Marowsky
Publisher: Springer Science & Business Media
Total Pages: 316
Release: 2012-12-06
Genre: Science
ISBN: 3642771947

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Progress made during the last few years in nonlinear optics and quantum electronics has significantly increased our understanding of the interactionbetween light and matter. Of great importance are third-order nonlinear Raman techniques such as CARS, RIKES, SRS, and DFWM. This book reflects the state of the art in coherent Raman spectroscopy. The contributions together provide an overview of the various Raman techniques that make available information about the fine structure of molecular energy levels, the collisional dynamics of atoms and molecules, and processes of internal energy disipation. Some of the contributions also report on the application of local, nonperturbing diagnosic methods forthe determination of parameters such as composition, temperature, density, velocity, and energy distribution between the internal degrees of freedom.

Application of Molecular Methods and Raman Microscopy/Spectroscopy in Agricultural Sciences and Food Technology

Application of Molecular Methods and Raman Microscopy/Spectroscopy in Agricultural Sciences and Food Technology
Author: Biljana Vucelić-Radović
Publisher: Ubiquity Press
Total Pages: 219
Release: 2019-07-23
Genre: Science
ISBN: 1911529544

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his book has been prepared with the aim to present the application of these two state-of-the art technologies in agricultural sciences and food technology, and to explain the protocols for analyses of different plant, animal, microbiological and food samples as well as for different biotechnology procedures. Selected methods and protocols which are used in plant stress physiology, weed science, fruit breeding research, microbial ecology, plant virus and fungus diagnostics, phytobacteriology, fishery, food biochemistry, food materials and food technology are described. Special adaptation of certain protocols is required for application in each of these sciences, for every type of GMO organism, food technology raw material, and food technology product, as well as for every type of bacteria, virus, fungus or fungus-like organism, for each type of raw material in terms of plant host species, plant organs, year period and conditions in the laboratory. Application of molecular methods, primarily qPCR, and Raman microscopy/ spectroscopy in agricultural and food sciences provides substantial opportunity for increased production efficiency, food safety, better product quality and improvement of plant and animal health. This book is aimed for students, scientists and professionals working in the field of agriculture and food technology.

Coherent Raman Spectroscopy

Coherent Raman Spectroscopy
Author: G. L. Eesley
Publisher: Elsevier
Total Pages: 153
Release: 2013-10-22
Genre: Science
ISBN: 1483153916

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Coherent Raman Spectroscopy provides a unified and general account of the fundamental aspects of nonlinear Raman spectroscopy, also known as coherent Raman spectroscopy. The theoretical basis from which coherent Raman spectroscopy developed is described, along with its applications, utility, and implementation as well as advantages and disadvantages. Experimental data which typifies each technique is presented. This book is comprised of four chapters and opens with an overview of nonlinear optics and coherent Raman spectroscopy, followed by a discussion on nonlinear transfer function of matter. The derivation of third-order nonlinear effects is described and the problem is specialized to one dimension by assuming monochromatic plane waves propagating in the z direction. The next chapter reviews the salient features of seven coherent Raman techniques by calculating the resonant and non-resonant signal contributions in the monochromatic plane wave limit. Finally, the general areas of application of coherent Raman spectroscopy are highlighted. This monograph is written primarily for uninitiated or endeavoring coherent Raman spectroscopists and should also be of interest to experienced industrial and academic researchers.