Super-Resolution Imaging in Biomedicine

Super-Resolution Imaging in Biomedicine
Author: Alberto Diaspro
Publisher: CRC Press
Total Pages: 375
Release: 2016-11-03
Genre: Science
ISBN: 1315355566
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This book encompasses the full breadth of the super-resolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in biomedicine. It shows readers how to use the new tools to increase resolution in sub-nanometer-scale images of living cells and tissue, which leads to new information about molecules, pathways and dynamics. The book highlights the advantages and disadvantages of the techniques, and gives state-of-the-art examples of applications using microscopes currently available on the market. It covers key techniques such as stimulated emission depletion (STED), structured illumination microscopy (SSIM), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM). It will be a useful reference for biomedical researchers who want to work with super-resolution imaging, learn the proper technique for their application, and simultaneously obtain a solid footing in other techniques.

Super-Resolution Imaging in Biomedicine

Super-Resolution Imaging in Biomedicine
Author: Alberto Diaspro
Publisher: CRC Press
Total Pages: 448
Release: 2016-11-03
Genre: Science
ISBN: 1482244357
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This book encompasses the full breadth of the super-resolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in biomedicine. It shows readers how to use the new tools to increase resolution in sub-nanometer-scale images of living cells and tissue, which leads to new information about molecules, pathways and dynamics. The book highlights the advantages and disadvantages of the techniques, and gives state-of-the-art examples of applications using microscopes currently available on the market. It covers key techniques such as stimulated emission depletion (STED), structured illumination microscopy (SSIM), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM). It will be a useful reference for biomedical researchers who want to work with super-resolution imaging, learn the proper technique for their application, and simultaneously obtain a solid footing in other techniques.

Standard and Super-Resolution Bioimaging Data Analysis

Standard and Super-Resolution Bioimaging Data Analysis
Author: Ann Wheeler
Publisher: John Wiley & Sons
Total Pages: 285
Release: 2017-10-12
Genre: Science
ISBN: 1119096936
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A comprehensive guide to the art and science of bioimaging data acquisition, processing and analysis Standard and Super-Resolution Bioimaging Data Analysis gets newcomers to bioimage data analysis quickly up to speed on the mathematics, statistics, computing hardware and acquisition technologies required to correctly process and document data. The past quarter century has seen remarkable progress in the field of light microscopy for biomedical science, with new imaging technologies coming on the market at an almost annual basis. Most of the data generated by these systems is image-based, and there is a significant increase in the content and throughput of these imaging systems. This, in turn, has resulted in a shift in the literature on biomedical research from descriptive to highly-quantitative. Standard and Super-Resolution Bioimaging Data Analysis satisfies the demand among students and research scientists for introductory guides to the tools for parsing and processing image data. Extremely well illustrated and including numerous examples, it clearly and accessibly explains what image data is and how to process and document it, as well as the current resources and standards in the field. A comprehensive guide to the tools for parsing and processing image data and the resources and industry standards for the biological and biomedical sciences Takes a practical approach to image analysis to assist scientists in ensuring scientific data are robust and reliable Covers fundamental principles in such a way as to give beginners a sound scientific base upon which to build Ideally suited for advanced students having only limited knowledge of the mathematics, statistics and computing required for image data analysis An entry-level text written for students and practitioners in the bioscience community, Standard and Super-Resolution Bioimaging Data Analysis de-mythologises the vast array of image analysis modalities which have come online over the past decade while schooling beginners in bioimaging principles, mathematics, technologies and standards.

Introductory Biomedical Imaging

Introductory Biomedical Imaging
Author: Bethe A. Scalettar
Publisher: CRC Press
Total Pages: 631
Release: 2022-09-08
Genre: Science
ISBN: 1351839977
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Imaging is everywhere. We use our eyes to see and cameras to take pictures. Scientists use microscopes and telescopes to peer into cells and out to space. Doctors use ultrasound, X-rays, radioisotopes, and MRI to look inside our bodies. If you are curious about imaging, open this textbook to learn the fundamentals. Imaging is a powerful tool in fundamental and applied scientific research and also plays a crucial role in medical diagnostics, treatment, and research. This undergraduate textbook introduces cutting-edge imaging techniques and the physics underlying them. Elementary concepts from electromagnetism, optics, and modern physics are used to explain prominent forms of light microscopy, as well as endoscopy, ultrasound, projection radiography and computed tomography, radionuclide imaging, and magnetic resonance imaging. This textbook also covers digital image processing and analysis. Theoretical principles are reinforced with illustrative homework problems, applications, activities, and experiments, and by emphasizing recurring themes, including the effects of resolution, contrast, and noise on image quality. Readers will learn imaging fundamentals, diagnostic capabilities, and strengths and weaknesses of techniques. This textbook had its genesis, and has been vetted, in a "Biomedical Imaging" course at Lewis & Clark College in Portland, OR, and is designed to facilitate the teaching of similar courses at other institutions. It is unique in its coverage of both optical microscopy and medical imaging at an intermediate level, and exceptional in its coverage of material at several levels of sophistication.

Super-Resolution Imaging

Super-Resolution Imaging
Author: Peyman Milanfar
Publisher: CRC Press
Total Pages: 490
Release: 2017-12-19
Genre: Computers
ISBN: 1439819319
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With the exponential increase in computing power and broad proliferation of digital cameras, super-resolution imaging is poised to become the next "killer app." The growing interest in this technology has manifested itself in an explosion of literature on the subject. Super-Resolution Imaging consolidates key recent research contributions from eminent scholars and practitioners in this area and serves as a starting point for exploration into the state of the art in the field. It describes the latest in both theoretical and practical aspects of direct relevance to academia and industry, providing a base of understanding for future progress. Features downloadable tools to supplement material found in the book Recent advances in camera sensor technology have led to an increasingly larger number of pixels being crammed into ever-smaller spaces. This has resulted in an overall decline in the visual quality of recorded content, necessitating improvement of images through the use of post-processing. Providing a snapshot of the cutting edge in super-resolution imaging, this book focuses on methods and techniques to improve images and video beyond the capabilities of the sensors that acquired them. It covers: History and future directions of super-resolution imaging Locally adaptive processing methods versus globally optimal methods Modern techniques for motion estimation How to integrate robustness Bayesian statistical approaches Learning-based methods Applications in remote sensing and medicine Practical implementations and commercial products based on super-resolution The book concludes by concentrating on multidisciplinary applications of super-resolution for a variety of fields. It covers a wide range of super-resolution imaging implementation techniques, including variational, feature-based, multi-channel, learning-based, locally adaptive, and nonparametric methods. This versatile book can be used as the basis for short courses for engineers and scientists, or as part of graduate-level courses in image processing.

Super-Resolution Microscopy

Super-Resolution Microscopy
Author: Holger Erfle
Publisher:
Total Pages:
Release: 2018-08-13
Genre:
ISBN: 9781493984350
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Imaging from Cells to Animals In Vivo

Imaging from Cells to Animals In Vivo
Author: Margarida Barroso
Publisher: CRC Press
Total Pages: 371
Release: 2020-12-03
Genre: Science
ISBN: 1351704508
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Imaging from Cells to Animals In Vivo offers an overview of optical imaging techniques developed over the past two decades to investigate biological processes in live cells and tissues. It comprehensively covers the main imaging approaches used as well as the application of those techniques to biological investigations in preclinical models. Among the areas covered are cell metabolism, receptor-ligand interactions, membrane trafficking, cell signaling, cell migration, cell adhesion, cytoskeleton and other processes using various molecular optical imaging techniques in living organisms, such as mice and zebrafish. Features Brings together biology and advanced optical imaging techniques to provide an overview of progress and modern methods from microscopy to whole body imaging. Fills the need for a comprehensive view of application-driven development and use of new tools to ask new biological questions in the context of a living system. Includes basic chapters on key methods and instrumentation, from fluorescence microscopy and imaging to endoscopy, optical coherence tomography and super-resolution imaging. Discusses approaches at different length scales and biomedical applications to the study of single cell, whole organ, and whole organism behavior. Addresses the impact on discovery, such as cellular function as implicated in human disease and translational medicine, for example in cancer diagnosis.

Label-Free Super-Resolution Microscopy

Label-Free Super-Resolution Microscopy
Author: Vasily Astratov
Publisher: Springer
Total Pages: 0
Release: 2020-09-20
Genre: Science
ISBN: 9783030217242
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This book presents the advances in super-resolution microscopy in physics and biomedical optics for nanoscale imaging. In the last decade, super-resolved fluorescence imaging has opened new horizons in improving the resolution of optical microscopes far beyond the classical diffraction limit, leading to the Nobel Prize in Chemistry in 2014. This book represents the first comprehensive review of a different type of super-resolved microscopy, which does not rely on using fluorescent markers. Such label-free super-resolution microscopy enables potentially even broader applications in life sciences and nanoscale imaging, but is much more challenging and it is based on different physical concepts and approaches. A unique feature of this book is that it combines insights into mechanisms of label-free super-resolution with a vast range of applications from fast imaging of living cells to inorganic nanostructures. This book can be used by researchers in biological and medical physics. Due to its logically organizational structure, it can be also used as a teaching tool in graduate and upper-division undergraduate-level courses devoted to super-resolved microscopy, nanoscale imaging, microscopy instrumentation, and biomedical imaging.

Microscopy Techniques for Biomedical Education and Healthcare Practice

Microscopy Techniques for Biomedical Education and Healthcare Practice
Author: Leonard Shapiro
Publisher: Springer Nature
Total Pages: 235
Release: 2023-11-02
Genre: Medical
ISBN: 3031368509
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This edited book has a strong focus on advances in microscopy that straddles research, medical education and clinical practice. These advances include the shift in power from conventional to digital microscopy. The first section of this book covers imaging techniques and morphometric image analysis with its applications in biomedicine using different microscopy modes. Chapters highlight the rich development of fluorescence methods and technologies; particle tracking techniques with applications in biomedical research and nanomedicine; the way in which visualizations have revolutionized taxonomy from gross anatomy to genetics; and the psychology of perception and how it affects our understanding of cells and tissues. The book’s first section concludes by exploring the use of CT modalities to evaluate anterior deformities in craniosynostosis. In the second section of the book, chapters on anatomical and cell biology education explore the history of anatomical models and their use in educational settings. This includes examples in 3D printing and functional human anatomical models that can be created using easily available resources and the use of biomedical imaging in visuospatial teaching of anatomy; the novel use of ultrasound in medical education and practice; and skill acquisition in histology education using a flowchart called a ‘decision tree’. This book will appeal to histologists, microscopists, cell biologists, clinicians and those involved in anatomical education and biomedical visualization, as well as students in those respective fields.

Nano-Optics

Nano-Optics
Author: Sabu Thomas
Publisher: Elsevier
Total Pages: 376
Release: 2020-07-06
Genre: Technology & Engineering
ISBN: 0128183934
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Nano-Optics: Fundamentals, Experimental Methods, and Applications offers insights into the fundamentals and industrial applications of nanoscale light-emitting materials and their composites. This book serves as a reference, offering an overview of existing research, with a particular focus on industrial applications. Nano-optics is the branch of nanoscience and nanotechnology that deals with interaction of light with nanoscale objects. This book explores the materials, structure, manufacturing techniques, and industrial applications of nano-optics. The applications discussed include healthcare, communication, astronomy, and satellites. Explains the major manufacturing techniques for light-emitting nanoscale materials Discusses how nanoscale optical materials are being used in a range of industrial applications Assesses the challenges of using nano-optics in a mass-production context