This volume contains the proceedings of the Workshop on In stability, Transition and Turbulence, sponsored by the Institute for Computer Applications in Science and Engineering (ICASE) and the NASA Langley Research Center (LaRC), during July 8 to August 2, 1991. This is the second workshop in the series on the subject. The first was held in 1989, and its proceedings were published by Springer-Verlag under the title "Instability and Transition" edited by M. Y. Hussaini and R. G. Voigt. The objectives of these work shops are to i) expose the academic community to current technologically im portant issues of transition and turbulence in shear flows over the entire speed range, ii) acquaint the academic community with the unique combination of theoretical, computational and experimental capabilities at LaRC and foster interaction with these capabilities, and iii) accelerate progress in elucidating the fundamental phenomena of transition and turbulence, leading to improved transition and turbulence modeling in design methodologies. The research areas covered in these proceedings include receptiv ity and roughness, nonlinear theories of transition, numerical simu lation of spatially evolving flows, modelling of transitional and fully turbulent flows as well as some experiments on instability and tran sition. In addition a one-day mini-symposium was held to discuss 1 recent and planned experiments on turbulent flow over a backward facing step.