Single Nucleotide Polymorphism Analysis By Matrix Assisted Laser Desorption Ionization Time Of Flight Mass Spectrometry
Download Single Nucleotide Polymorphism Analysis By Matrix Assisted Laser Desorption Ionization Time Of Flight Mass Spectrometry full books in PDF, epub, and Kindle. Read online free Single Nucleotide Polymorphism Analysis By Matrix Assisted Laser Desorption Ionization Time Of Flight Mass Spectrometry ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Author | : Zhengdong Fei |
Publisher | : |
Total Pages | : 350 |
Release | : 1999 |
Genre | : |
ISBN | : |
Download Single Nucleotide Polymorphism Analysis by Matrix-assisted Laser Desorption/ Ionization Time-of-flight Mass Spectrometry Book in PDF, Epub and Kindle
Author | : Yongdong Zhu |
Publisher | : |
Total Pages | : 364 |
Release | : 1999 |
Genre | : |
ISBN | : |
Download Fundamental Studies of Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry (MALDI-TOF/MS) and Its Applications to the Analysis of Biological Materials Book in PDF, Epub and Kindle
Author | : Rachel Jennifer Ball |
Publisher | : |
Total Pages | : 191 |
Release | : 2005 |
Genre | : |
ISBN | : |
Download A mass spectrometry based hybridisation assay for single nucleotide polymorphism analysis Book in PDF, Epub and Kindle
Author | : J.N. Housby |
Publisher | : Springer Science & Business Media |
Total Pages | : 161 |
Release | : 2001-10-31 |
Genre | : Medical |
ISBN | : 0792371739 |
Download Mass Spectrometry and Genomic Analysis Book in PDF, Epub and Kindle
The human genome project is set to revolutionise health care and medicine in the near future. The genetic make up of each individual gives clues as to the genetic factors that predispose one to a particular genetic disease. Single Nucleotide Polymorphisms (SNPs), single base changes in the nucleotide DNA sequence of individuals, are thought to be the main cause of genetic variation. By comparing patterns of SNP allele frequencies between disease affected and control populations, disease associated SNPs can be identified and potential disease gene(s) located. These types of study necessitate genotyping of thousands of SNPs which requires the use of powerful, high throughput, systems of analysis. Mass spectrometry is fast becoming the preferred technology for this type of high throughput analysis. This book contains a collection of descriptions of some of the most outstanding advances in the field of mass spectrometry from which, I hope, the reader will be able to learn both the principles and the most up to date methods for its use in genomic analysis. It covers the general principles of the technologies and more in depth detailed descriptions for more academic reading and information. Audience: Whether you are a student, a post-doctoral researcher or experienced MS user, this book will be a stimulating addition to the analytical arena of mass spectrometry and genomic analysis.
Author | : Pui-Yan Kwok |
Publisher | : Springer Science & Business Media |
Total Pages | : 276 |
Release | : 2008-02-04 |
Genre | : Medical |
ISBN | : 1592593275 |
Download Single Nucleotide Polymorphisms Book in PDF, Epub and Kindle
A comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.
Author | : Robin Liu |
Publisher | : Springer Science & Business Media |
Total Pages | : 216 |
Release | : 2008-12-03 |
Genre | : Medical |
ISBN | : 0387767592 |
Download Integrated Biochips for DNA Analysis Book in PDF, Epub and Kindle
The objective of this book is to provide up-to-date coverage of some of the emerging developments in the field of integrated DNA biochips. It will prove a useful source of information for researchers in the field and for those who are just entering the field of biochip research.
Author | : |
Publisher | : |
Total Pages | : 11 |
Release | : 1995 |
Genre | : |
ISBN | : |
Download Laser Desorption Mass Spectrometry for DNA Analysis and Sequencing Book in PDF, Epub and Kindle
Laser desorption mass spectrometry has been considered as a potential new method for fast DNA sequencing. Our approach is to use matrix-assisted laser desorption to produce parent ions of DNA segments and a time-of-flight mass spectrometer to identify the sizes of DNA segments. Thus, the approach is similar to gel electrophoresis sequencing using Sanger's enzymatic method. However, gel, radioactive tagging, and dye labeling are not required. In addition, the sequencing process can possibly be finished within a few hundred microseconds instead of hours and days. In order to use mass spectrometry for fast DNA sequencing, the following three criteria need to be satisfied. They are (1) detection of large DNA segments, (2) sensitivity reaching the femtomole region, and (3) mass resolution good enough to separate DNA segments of a single nucleotide difference. It has been very difficult to detect large DNA segments by mass spectrometry before due to the fragile chemical properties of DNA and low detection sensitivity of DNA ions. We discovered several new matrices to increase the production of DNA ions. By innovative design of a mass spectrometer, we can increase the ion energy up to 45 KeV to enhance the detection sensitivity. Recently, we succeeded in detecting a DNA segment with 500 nucleotides. The sensitivity was 100 femtomole. Thus, we have fulfilled two key criteria for using mass spectrometry for fast DNA sequencing. The major effort in the near future is to improve the resolution. Different approaches are being pursued. When high resolution of mass spectrometry can be achieved and automation of sample preparation is developed, the sequencing speed to reach 500 megabases per year can be feasible.
Author | : Alexander R. Ivanov |
Publisher | : Springer Science & Business Media |
Total Pages | : 1081 |
Release | : 2011-06-15 |
Genre | : Science |
ISBN | : 9400708289 |
Download Sample Preparation in Biological Mass Spectrometry Book in PDF, Epub and Kindle
The aim of this book is to provide the researcher with important sample preparation strategies in a wide variety of analyte molecules, specimens, methods, and biological applications requiring mass spectrometric analysis as a detection end-point. In this volume we have compiled the contributions from several laboratories which are employing mass spectrometry for biological analysis. With the latest inventions and introduction of highly sophisticated mass spectrometry equipment sample preparation becomes an extremely important bottleneck of biomedical analysis. We have a goal of giving the reader several successful examples of sample preparation, development and optimization, leading to the success in analytical steps and proper conclusions made at the end of the day. This book is structured as a compilation of contributed chapters ranging from protocols to research articles and reviews. The main philosophy of this volume is that sample preparation methods have to be optimized and validated for every project, for every sample type and for every downstream analytical technique.
Author | : Jose V. Bonilla |
Publisher | : CRC Press |
Total Pages | : 502 |
Release | : 2011-02-23 |
Genre | : Medical |
ISBN | : 1439819947 |
Download Handbook of Analysis of Oligonucleotides and Related Products Book in PDF, Epub and Kindle
Oligonucleotides represent one of the most significant pharmaceutical breakthroughs in recent years, showing great promise as diagnostic and therapeutic agents for malignant tumors, cardiovascular disease, diabetes, viral infections, and many other degenerative disorders. The Handbook of Analysis of Oligonucleotides and Related Products is an essen
Author | : Hassan Rashidzadeh |
Publisher | : |
Total Pages | : 230 |
Release | : 1999 |
Genre | : Electron-stimulated desorption |
ISBN | : |
Download Study of Matrix-assisted Laser Desorption/ionization Time of Flight Mass Spectrometry for Polymer Analysis Book in PDF, Epub and Kindle