Serum/Plasma Proteomics

Serum/Plasma Proteomics
Author: David W. Greening
Publisher: Springer Nature
Total Pages: 591
Release: 2023-02-13
Genre: Science
ISBN: 1071629786

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This third volume provides comprehensive protocols on pre-analytical, analytical, plasma, and serum proteomics. New and updated chapters are divided into nine sections, detailing blood processing and handling strategies, discovery- and targeted-based mass spectrometry, including workflows to aid in discovery and targeted data analysis, in addition to software and bioinformatics for the plasma proteome. This edition further integrates emerging areas in the development of technologies for plasma proteomics and assay platforms in biomarker discovery and translational proteomics, enrichment and detection strategies to understand the plasma proteome, and peptide, lipid and metabolite targeted assays. We also detail the emerging analysis of extracellular vesicles isolated from plasma. Written in the format of the highly successful Methods in Molecular Biology series, each of the 33 chapters includes an introduction to the topic, lists necessary materials and methods, includes hints and tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Serum/Plasma Proteomics: Methods and Protocols, Third Edition aims to be comprehensive guide for researchers.

Serum/Plasma Proteomics

Serum/Plasma Proteomics
Author: Richard J Simpson
Publisher: Humana Press
Total Pages: 412
Release: 2016-05-01
Genre:
ISBN: 9781493958016

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Blood science has become a cornerstone of multiple disciplines. This book, contributed to by leading experts in the field, provides a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics.

Emerging Sample Treatments in Proteomics

Emerging Sample Treatments in Proteomics
Author: José-Luis Capelo-Martínez
Publisher: Springer
Total Pages: 222
Release: 2019-06-24
Genre: Science
ISBN: 3030122980

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Proteomics is a well-established area of Science; yet with a strong area in constant evolution, namely sample treatment. There few books that currently cover the field of emerging sample treatments in proteomics, this new volume will be the first to cover all emerging and existing studies. This unique book presents the latest advances in the field focusing on emerging trends linked to high-resolution mass spectrometry, technology addressed to treat samples faster and to attempts to simplify the proteome for the reader.

Quantitative Proteome Analysis

Quantitative Proteome Analysis
Author: Kazuhiro Imai
Publisher: CRC Press
Total Pages: 314
Release: 2013-08-05
Genre: Science
ISBN: 9814316512

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This book focuses on the advantages and disadvantages of each of the commonly used quantitative proteomic methods in terms of accuracy, sensitivity, and reproducibility. It also concentrates on the effective applications of these methods that resulted in many discoveries of the role of the proteins expressed in living cells and biological fluids. The first part of the book focuses on the description of advantages and disadvantages of each of the commonly used quantitative proteomic methods in terms of accuracy, sensitivity, and, especially, reproducibility. The second part of the book focuses on providing concise descriptions of the effective applications of these methods to demonstrate how they have resulted in many important discoveries of the roles of the proteins expressed in living cells.

Exploring the Human Plasma Proteome

Exploring the Human Plasma Proteome
Author: Gilbert S. Omenn
Publisher: John Wiley & Sons
Total Pages: 394
Release: 2006-12-13
Genre: Science
ISBN: 3527609423

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On the cutting edge of medical diagnostics, plasma proteomics promises to generate a new wave of technologies to help identify many different diseases and disease risks. Plasma and serum are the preferred non-invasive specimens to test normal individuals, at-risk groups, and patients for protein biomarkers discovered and validated to reflect physiological, pathological, and pharmacological phenotypes. These specimens present enormous challenges due to extreme complexity, huge dynamic range in protein concentrations, non-standardized methods of sample processing, and intra- and inter-individual variation from genetics, diet, smoking, hormones, and other sources. This book presents the major findings from the collaborative Plasma Proteome Project organized by the international Human Proteome Organization (HUPO). The chapters are drawn from a larger set of publications in the journal PROTEOMICS. This book provides a valuable foundation for development and applications of proteomics.

The Proteomics Protocols Handbook

The Proteomics Protocols Handbook
Author: John M. Walker
Publisher: Springer Science & Business Media
Total Pages: 969
Release: 2007-10-09
Genre: Science
ISBN: 1592598900

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Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Proteomics of Human Body Fluids

Proteomics of Human Body Fluids
Author: Visith Thongboonkerd
Publisher: Springer Science & Business Media
Total Pages: 536
Release: 2008-01-23
Genre: Science
ISBN: 159745432X

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This volume is the first collection of applications of proteomics to analyze various human body fluids. Proteomics of Human Bodyfluids consists of two parts. The first provides basic principles and strategies for proteomic analysis of human body fluids. The second offers more details regarding methodologies and recent findings and clinical applications of each specific type of human body fluids.

A Century of Separation Science

A Century of Separation Science
Author: Haleem J. Issaq
Publisher: CRC Press
Total Pages: 782
Release: 2001-12-19
Genre: Science
ISBN: 9780824705763

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A Century of Separation Science presents an extensive overview of the critical developments in separation science since 1900, covering recent advances in chromatography, electrophoresis, field-flow fractionation, countercurrent chromatography, and supercritical fluid chromatography for high-speed and high-throughput analysis.

Proteomics for Biomarker Discovery

Proteomics for Biomarker Discovery
Author: Ming Zhou
Publisher: Humana
Total Pages: 0
Release: 2016-08-23
Genre: Science
ISBN: 9781493959990

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With the advent of proteomics came the development of technologies, primarily mass spectrometry, which allowed high-throughput identification of proteins in complex mixtures. While the mass spectrometer resides at the heart of proteomics, its ability to characterize biological samples is only as good as the sample preparation and data analysis tools used in any study. In Proteomics for Biomarker Discovery, expert researchers in the field detail many of the methods which are now commonly used to study proteomics. These include methods and techniques include both label-free approaches and those that utilize stable isotopes incorporated both during cell growth or added via a chemical reaction once the proteome is extracted from the cell. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Proteomics for Biomarker Discovery seeks to aid scientists in the further study the different sample preparation and data analysis tools used in proteomics today.