Mammals in freshwater aquatic systems play important roles as ecosystem engineers, trophic transfer agents, and apex predators, thus acting as indicators of freshwater ecosystem function. Watersheds inhabited by semi-aquatic mammals have increased links between adjacent terrestrial and aquatic ecosystems compared to watersheds where they are not present. Semiaquatic mammals not only exert top-down influences on streams, but are affected by bottom-up forces from the riparian system itself. The goal of this study was to identify variables that correlate with the presence of beaver (Castor canadensis), muskrat (Ondatra zibethicus ), mink (Neovison vison), and river otter ( Lontra canadensis), including their interactions, resulting in a better understanding of the areas where these semi-aquatic mammals occur and their effects on the riparian system. The objectives of this study were (1) to identify variables related to the probability of detection, initial occupancy, colonization, and extinction of the 4 semi-aquatic mammals in southern Illinois; and (2) to test if the reintroduction of river otter has changed stream food webs. To address my first objective, I sampled 120 bridge sites in 2 periods (winter: Jan-Feb; and spring: Mar-Apr) during 2012–2014 in 11 major watersheds in the southern third of Illinois (44,526 km2) to estimate multi-season occupancy. Each survey unit was a 400-m stream segment visited twice by 2 observers for a total of 4 observations per site per period. Observers recorded all mammal signs found, including sign species and type. Sites were Intensive Basin Survey Sites sampled by Illinois Department of Natural Resources (IDNR) and Illinois Environmental Protection Agency (IEPA), allowing data collected by the state to be available for explanatory variables for mammal occupancy. Data collected by the Illinois Natural History Survey (INHS) also were available for a subset of sites. I collected local- and landscape-scale habitat and weather variables for each site. I developed hypotheses regarding occupancy of sites based on land-cover, human disturbance, and stream attributes for each species. I developed additional hypotheses regarding prey availability and water quality for river otter and mink. Sites used in each analysis were dependent on data available to address the hypotheses of interest. My results indicate that semi-aquatic mammals in Illinois were affected by a riparian habitat, water availability, and stream community variables at both the landscape and local scale. I found high occupancy of mink, beaver, and muskrat across the entire landscape of southern Illinois, and my results suggest that the geographic range of river otter continues to expand. Relationships of occupancy of these semi-aquatic mammals to measurements of urban areas and human disturbance were not consistent across all species. Mink and river otter occupancy were both predicted by aspects of prey availability, indicating the importance of predator-prey relationships in occupancy dynamics of riparian predators. Hypotheses regarding predator pressure and changes in environmental variables were used to test the effects of river otter reintroduction on stream communities. For this objective, I used structural equation models. I compared fish and macroinvertebrate communities from before (1982-1995) and after (2005-2013) reintroduction of river otter, which occurred in 1994–1996. Fish and macroinvertebrate community data for 35 sites located throughout 6 major watersheds in southern Illinois (25,550 km2) were obtained from state agencies. Changes in stream communities were evaluated using 4 metrics (species richness, species dominance, skewness in size distribution of prey, and proportion of individuals in the size class preferred by river otter). Neither the inclusion of river otter site use nor change in stream quality, measured by change in % forest, improved models over the simple model which only included fish and macroinvertebrate communities. Overall, I found no evidence that river otter presence or change in forest cover affected stream fish and macroinvertebrate communities. (Abstract shortened by ProQuest.)