Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies

Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies
Author: Anna Porwit
Publisher: Cambridge University Press
Total Pages: 248
Release: 2018-01-25
Genre: Medical
ISBN: 1108548830

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Master implementation of the techniques of flow cytometry in diagnosing complex haematological diseases and malignancies in patients, worldwide. Featuring World Health Organization recommendations on pre-analytical steps, instrument settings and panel construction, this invaluable manual offers invaluable support for those researching, practising and analyzing the cause of hematological malignancies. Authored by leading experts, this book puts flow-cytometry into everyday context. With a focus on multicolour panels, the manual provides readers an experienced understanding of effective, implementation techniques. Practitioners of all levels are offered a background in a variety of diseases presented alongside the most current methodology. Wide-ranging and comprehensive; detailed images of healthy blood, bone marrow and lymph-nodes are illustrated throughout, allowing for effective diagnosis. Through engaging with differential diagnoses, the manual offers an understanding of similar symptoms and mimicking malignancies, avoiding inaccurate results. Featuring in-depth descriptions of chronic diseases; users can reach accurate diagnosis, first time.

Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies

Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies
Author: Anja Porwit
Publisher: Cambridge University Press
Total Pages: 247
Release: 2018-01-25
Genre: Medical
ISBN: 1107503833

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This practical manual offers an active understanding of how to implement flow-cytometry when facing complex, haematological diseases.

Flow Cytometry of Hematological Malignancies

Flow Cytometry of Hematological Malignancies
Author: Claudio Ortolani
Publisher: John Wiley & Sons
Total Pages: 464
Release: 2021-04-19
Genre: Medical
ISBN: 111961130X

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Flow Cytometry of Hematological Malignancies Flow cytometric analysis is often integral to the swift and accurate diagnosis of leukemias and lymphomas of the blood, bone marrow, and lymph nodes. However, in the fast-moving and expanding field of clinical hematology, in can be challenging to remain up to speed with the latest biological research and technological innovations. Flow Cytometry of Hematological Malignancies has been designed to provide all those working in hematological oncology with a practical, cutting-edge handbook, featuring clear and fully illustrated guidance on all aspects of cytometry’s role in diagnosis and analysis. This essential second edition includes: Explorations of more than 70 antigens Full-color illustrations throughout New descriptions of recently discovered markers WHO classifications of hematological neoplastic diseases Helpful tips for result interpretation and analysis Featuring all this and more, Flow Cytometry of Hematological Malignancies, Second Edition, is an invaluable resource for both trainee and experienced hematologists, hematopathologists, oncologists, and pathologists, as well as medical students and diagnostic lab technicians.

Flow Cytometry, Immunohistochemistry, and Molecular Genetics for Hematologic Neoplasms

Flow Cytometry, Immunohistochemistry, and Molecular Genetics for Hematologic Neoplasms
Author: Tsieh Sun
Publisher: Lippincott Williams & Wilkins
Total Pages: 449
Release: 2012-01-19
Genre: Medical
ISBN: 1451180640

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Immunophenotyping is the most powerful tool in the routine diagnosis of hematologic neoplasms. Immunohistochemical technique is used in histology labs for this purpose, while flow cytometry is used in clinical labs. Although separately these 2 techniques are very useful in detecting lymphomas and leukemias, the combination of both creates a very powerful and definitive diagnostic tool. The addition of molecular genetics to the book makes it an all-encompassing reference text.

Minimal Residual Disease in Hematologic Malignancies

Minimal Residual Disease in Hematologic Malignancies
Author: Raanani P Ed
Publisher: S. Karger AG (Switzerland)
Total Pages: 0
Release: 2004
Genre: Blood
ISBN: 9783805577731

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Detection of minimal residual disease (MRD) is increasingly used in the management of leukemia patients. A wide variety of methods have been developed and include technologies designed to detect residual malignant cells beyond the sensitivity of conventional approaches such as morphology and banding cytogenetics in leukemia. The choice of the best method depends on the biology of the individual malignancy, i.e. on the determination of specific markers which are useful to differentiate between leukemic cells and normal hematopoiesis in leukemic patients. These markers include leukocyte differentiation antigens, fusion transcripts, transcripts overexpressed by mutated or nonmutated genes, rearranged genes, and individual markers like polymorphic repetitive DNA sequences. The major technologies for MRD detection, their advantages and disadvantages and their clinical applications are discussed in this special issue - from 'bench to bedside'. Providing a comprehensive overview on the significance of MRD in the evaluation, treatment and follow-up of hematologic malignancies, it will be of great value to hematologists, researchers interested in leukemias and lymphomas as well as laboratory technicians.

Laboratory Hematology Practice

Laboratory Hematology Practice
Author: Kandice Kottke-Marchant
Publisher: John Wiley & Sons
Total Pages: 777
Release: 2012-06-06
Genre: Medical
ISBN: 1444398571

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Expertly edited and endorsed by the International Society for Laboratory Hematology, this is the newest international textbook on all aspects of laboratory hematology. Covering both traditional and cutting-edge hematology laboratory technology this book emphasizes international recommendations for testing practices. Illustrative case studies on how technology can be used in patient diagnosis are included. Laboratory Hematology Practice is an invaluable resource for all those working in the field.

Flow Cytometry in Neoplastic Hematology

Flow Cytometry in Neoplastic Hematology
Author: Wojciech Gorczyca
Publisher: CRC Press
Total Pages: 350
Release: 2010-02-25
Genre: Medical
ISBN: 1841847445

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This highly illustrated, practical guide contains comprehensive coverage of all the important factors for clinical diagnosis with flow cytometry. It explains the general parameters and correlation with color histomorphological findings throughout, taking a systematic approach from basic cases to complicated problem areas. Hematopathologists and neoplastic hematologists will find this book an important resource for keeping up to date with developments in clinical practice. This second edition includes a chapter on antigen expression during myeloid and lymphoid differentiation.

Flow Cytometry in Hematology

Flow Cytometry in Hematology
Author: Ole Didrik Laerum
Publisher:
Total Pages: 304
Release: 1992
Genre: Medical
ISBN:

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This book reviews flow cytometric methods (techniques for measuring and sorting of cells) used in hematology--ranging from those in routine use (such as leukocyte counting and immunophentotyping in diseases like leukemia and AIDS) to those that have potential future use in experimental and clinical hematology. This volume will be of interest to a wide audience, including cell biologists, hematologists, cancer researchers, and HIV/AIDS researchers.

Practical Flow Cytometry in Haematology

Practical Flow Cytometry in Haematology
Author: Mike Leach
Publisher: John Wiley & Sons
Total Pages: 415
Release: 2015-07-20
Genre: Medical
ISBN: 1118747038

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The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis. This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors’ institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis. The reader will review May−Grünwald−Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference. Practical Flow Cytometry in Haematology: 100 Worked Examples: Provides a practical, example-based resource for flow cytometry Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care Includes both malignant and benign conditions Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207) Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations.

Pediatric Lymphomas

Pediatric Lymphomas
Author: Howard J. Weinstein
Publisher: Springer Science & Business Media
Total Pages: 296
Release: 2007-01-20
Genre: Medical
ISBN: 354068753X

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This is a comprehensive textbook of Hodgkin's and non-Hodgkin's lymphomas written by leaders in the field of childhood lymphomas. It includes clinical, pathologic and molecular biology of each subtype of lymphoma. The pathology chapters are comprehensive and include excellent photographs. The book is at the level of subspecialists in pediatric hematology and oncology, radiation oncology, pediatric surgery and hematopathology.