The lateral ankle sprain (LAS) is the most common orthopedic injury in athletics and is the result of simultaneous excessive inversion of the ankle and supination of the foot. Following initial LAS, degenerative changes have been found in over 70% of participants for extended periods of time. With the aforementioned degenerative changes typically come alterations in movement patterns, muscle activation patterns, and arthrokinematics. The current study aimed to investigate the effects of LAS history on neuromuscular control during a soccer specific jump-landing task and to illustrate the impact of limb (injured vs. non-injured) on neuromuscular control during a soccer specific jump-landing task in female adolescent athletes (n = 14, age = 16 ± 1.0 yr). A control group (n = 7) and a group designated as the "ankle sprain" group (n = 7) were used. The injured ankles in the ankle sprain group and the dominant limbs of the control group were labeled "affected," while the uninjured limbs of the ankle sprain group and the non-dominant limbs of the control group were labeled "unaffected." Electromyography (EMG) sensors were placed on 6 lower-limb muscles of each participant bilaterally, totaling 12 sensors per participant -- Lateral Gastrocnemius (LG), Peroneus Longus (PL), Tibialis Anterior (TA), Vastus Lateralis (VL), Vastus Medialis (VM), and Biceps Femoris (BF). Each participant completed a 5-minute low-to-moderate intensity warm-up on a cycler ergometer, then completed 10 soccer-header jump-landing trials. There were no significant limb by group interactions (p > 0.05) or group main effects (p > 0.05) found in the current study. Pre-landing data showed significant limb main effects for the vi standard deviation (SD) of the unaffected PL (p = 0.017) and the affected VL (p = 0.048). Furthermore, significant limb main effects were discovered for the COV of pre-landing muscle activation for the PL (p = 0.043), LG (p = 0.049), and VL (p = 0.048). During the post-landing period, significant limb main effects were recorded for the average activation (p = 0.001) and SD (p = 0.003) of the PL and the COV of the VL (p = 0.038). Reduced activation of the nondominant limbs illustrated increased neuromuscular control in the distal musculature (PL), while the proximal musculature (VL) demonstrated reduced neuromuscular control on the unaffected limbs. The current study discovered that limb dominance was a more prominent factor in altered lower-leg neuromuscular control compared to ankle injury history. Limb differences were most likely the result of the sport-specific demands placed on the dominant and non-dominant limbs during soccer, which elicited increased muscular responses of the non-dominant limb during jump-landing tasks.