Maximizing Gene Expression

Maximizing Gene Expression
Author: William Reznikoff
Publisher: Elsevier
Total Pages: 390
Release: 2014-05-20
Genre: Science
ISBN: 1483100804

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Maximizing Gene Expression focuses on prokaryotic and eukaryotic gene expression. The book first discusses E. coli promoters. Topics include structure analysis, steps in transcription initiation, structure-function correlation, and regulation of transcription initiation. The text also highlights yeast promoters, including elements that select initiation sites, transcription regulation, regulatory proteins, and upstream promoter elements. The text also describes protein coding genes of higher eukaryotes; instability of messenger RNA in bacteria; and replication control of the ColE1-type plasmids. The text then describes translation initiation, including the translation of prokaryotes and eukaryotes. The book puts emphasis on the selective degradation of abnormal proteins in bacteria. Topics include proteins rapidly hydrolyzed in E. coli; intracellular aggregates of abnormal polypeptides; energy requirement and pathway for proteins; proteolytic enzymes in E. coli; and regulation of ion expression. The text also highlights the detection of proteins produced by recombinant DNA techniques and mechanism and practice. The book is a good source of information for readers wanting to study gene expression.

Gene Expression Technology

Gene Expression Technology
Author: David V. Goeddel
Publisher:
Total Pages: 728
Release: 1990
Genre: Gene Expression
ISBN:

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The methods presented in this volume will enable the reader to design effective strategies for the expression of cloned genes and cDNAs and will prove useful in solving the majority of expression problems one is likely to encounter.

Gene Expression Systems

Gene Expression Systems
Author: Joseph M. Fernandez
Publisher: Elsevier
Total Pages: 493
Release: 1998-12-21
Genre: Science
ISBN: 0080532357

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Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. Provides detailed information on expression systems Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs Includes timelines and examples Compares pros and cons of each method

Regulation of gene expression

Regulation of gene expression
Author: U Satyanarayana
Publisher: Elsevier Health Sciences
Total Pages: 25
Release: 2014-11-07
Genre: Medical
ISBN: 8131241831

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Regulation of gene expression Regulation of gene expression

Recombinant Gene Expression

Recombinant Gene Expression
Author: Paulina Balbas
Publisher: Springer Science & Business Media
Total Pages: 505
Release: 2008-02-04
Genre: Science
ISBN: 1592597742

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Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or worse yet, for being occasionally dangerous and pathogenic. H- ever, it was easily identified in contaminated waters during the 19th century, thus becoming a clear indicator of water pollution by human feces. Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation, and further on—gene expression. Although at the lower end of the complexity scale, this microbe became a very successful model system and a key player in the fantastic revolution kindled by the birth of recombinant DNA technology.

Regulation of Gene Expression

Regulation of Gene Expression
Author: Gary H. Perdew
Publisher: Springer Science & Business Media
Total Pages: 334
Release: 2008-08-17
Genre: Science
ISBN: 1597452289

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The use of molecular biology and biochemistry to study the regulation of gene expression has become a major feature of research in the biological sciences. Many excellent books and reviews exist that examine the experimental methodology employed in specific areas of molecular biology and regulation of gene expression. However, we have noticed a lack of books, especially textbooks, that provide an overview of the rationale and general experimental approaches used to examine chemically or disease-mediated alterations in gene expression in mammalian systems. For example, it has been difficult to find appropriate texts that examine specific experimental goals, such as proving that an increased level of mRNA for a given gene is attributable to an increase in transcription rates. Regulation of Gene Expression: Molecular Mechanisms is intended to serve as either a textbook for graduate students or as a basic reference for laboratory personnel. Indeed, we are using this book to teach a graduate-level class at The Pennsylvania State University. For more details about this class, please visit http://moltox. cas. psu. edu and select “Courses. ” The goal for our work is to provide an overview of the various methods and approaches to characterize possible mechanisms of gene regulation. Further, we have attempted to provide a framework for students to develop an understanding of how to determine the various mechanisms that lead to altered activity of a specific protein within a cell.

Translational Control of Gene Expression

Translational Control of Gene Expression
Author: Nahum Sonenberg
Publisher: CSHL Press
Total Pages: 1034
Release: 2001
Genre: Gene expression
ISBN: 9780879696184

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Since the 1996 publication of Translational Control, there has been fresh interest in protein synthesis and recognition of the key role of translation control mechanisms in regulating gene expression. This new monograph updates and expands the scope of the earlier book but it also takes a fresh look at the field. In a new format, the first eight chapters provide broad overviews, while each of the additional twenty-eight has a focus on a research topic of more specific interest. The result is a thoroughly up-to-date account of initiation, elongation, and termination of translation, control mechanisms in development in response to extracellular stimuli, and the effects on the translation machinery of virus infection and disease. This book is essential reading for students entering the field and an invaluable resource for investigators of gene expression and its control.

Eucaryotic Gene Regulation

Eucaryotic Gene Regulation
Author: Richard Axel
Publisher: Elsevier
Total Pages: 682
Release: 2012-12-02
Genre: Science
ISBN: 0323143504

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Eukaryotic Gene Regulation covers the aspects and mechanisms of gene regulation of selected eukaryotes, such as yeast, Drosophila, and insect. This book is organized into eight parts, encompassing 52 chapters. The majority of the chapters are presented in an experimental manner containing an abstract, methods, results and discussion, and conclusion. This book first gives a short overview of the evolutionary role of interspersion in eukaryotic genes. It then presents considerable chapters on control of gene expression in yeast; gene mutation and isolation; structure and function; and analysis. Part III focuses on genetic and DNA sequence analysis in Drosophila. It includes discussions on allelic complementation and transvection, genetic organization, histone gene, and gene transcription. Part IV examines cell lineage; gene expression and sequences; and protein synthesis of insects, sea urchin, and mammalian cells. This is followed by discussions on structure and expression of specific eukaryotic genes from chicken, rat, rabbit, and human. Topics on the transfer of genetic information within and between cells and the structure and function of chromosome are significantly considered in Parts VI and VII. Genes evaluated in these sections include heavy chain immunoglobulin, light chain, beta-globin, and dihydrofolate reductase. Furthermore, this book describes the in vitro transcription and the factors involved; internal organization and mechanism of assembly of nucleosome; and chromatin structure. The concluding section focuses on aspects of viral genome expression including gene regulation, synthesis, processing, and alternative RNA splicing. Research biologists, geneticists, scientists, teachers, and students will greatly benefit from this book.

Gene Expression and Regulation in Mammalian Cells

Gene Expression and Regulation in Mammalian Cells
Author: Fumiaki Uchiumi
Publisher: BoD – Books on Demand
Total Pages: 374
Release: 2018-02-28
Genre: Medical
ISBN: 9535138677

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Sixty years after the "central dogma," great achievements have been developed in molecular biology. We have also learned the important functions of noncoding RNAs and epigenetic regulations. More importantly, whole genome sequencing and transcriptome analyses enabled us to diagnose specific diseases. This book is not only intended for students and researchers working in laboratory but also physicians and pharmacists. This volume consists of 14 chapters, divided into 4 parts. Each chapter is written by experts investigating biological stresses, epigenetic regulation, and functions of transcription factors in human diseases. All articles presented in this volume by excellent investigators provide new insights into the studies in transcriptional control in mammalian cells and will inspire us to develop or establish novel therapeutics against human diseases.