Light scattering properties of cells
Author | : Andrew Kenneth Dunn |
Publisher | : |
Total Pages | : 396 |
Release | : 1997 |
Genre | : Light |
ISBN | : |
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Author | : Andrew Kenneth Dunn |
Publisher | : |
Total Pages | : 396 |
Release | : 1997 |
Genre | : Light |
ISBN | : |
Author | : Marina Moran |
Publisher | : |
Total Pages | : 142 |
Release | : 2013 |
Genre | : Cells |
ISBN | : |
The scattered light pattern from a biological cell is greatly influenced by the internal structure and optical properties of the cell. This research project examines the relationships between the morphological and scattering properties of biological cells through numerical simulations. The mains goals are: (1) to develop a procedure to analytically model biological cells, (2) to quantitatively study the effects of a range of cell characteristics on the features of the light scattering patterns, and (3) to classify cells based on the features of their light scattering patterns. A procedure to create an analytical cell model was developed which extracted structural information from the confocal microscopic images of cells and allowed for the alteration of the cell structure in a controlled and systematic way. The influence of cell surface roughness, nuclear size, and mitochondrial volume density, spatial distribution, size and shape on the light scattering patterns was studied through numerical simulations of light scattering using the Discrete Dipole Approximation. It was found that the light scattering intensity in the scattering angle range of 25° to 45° responded to changes in the surface fluctuation of the cell and the range of 90° to 110° was well suited for characterization of mitochondrial density and nuclear size. A comparison of light scattering pattern analysis methods revealed that the angular distribution of the scattered light and Gabor filters were most helpful in differentiating between the cell characteristics. In addition, a measured increase in the Gabor energy of the light scattering patterns in response to an increase in the complexity of the cell models suggested that a complex nuclear structure and mitochondria should be included when modeling biological cells for light scattering simulations. Analysis of the scattering pattern features with Gabor filters resulted in discrimination of the cell models according to cell surface roughness, nuclear size, and mitochondrial volume density and size with over 90% classification accuracy. This study suggested the location of the scattering planes that are most relevant to researchers depending on the desired information about the cell and may provide a quantitative approach to cell discrimination with practical applications in flow cytometry for the diagnosis of diseases.
Author | : |
Publisher | : |
Total Pages | : |
Release | : 2014 |
Genre | : |
ISBN | : |
Author | : Bobby Eugene Pyle |
Publisher | : |
Total Pages | : 302 |
Release | : 1978 |
Genre | : Light |
ISBN | : |
Author | : D. W. Ross |
Publisher | : Springer |
Total Pages | : 338 |
Release | : 1981-02-01 |
Genre | : Medical |
ISBN | : 9783540102250 |
Author | : Alexander Kokhanovsky |
Publisher | : Springer |
Total Pages | : 390 |
Release | : 2019-06-29 |
Genre | : Science |
ISBN | : 3030205878 |
This book describes recent advances in radiative transfer, atmospheric remote sensing, polarization optics of random media, and light scattering. It is a valuable resource for anyone involved in light scattering research. Providing numerous step-by-step tutorials, it allows readers to quickly learn about various aspects of theoretical and experimental light scattering media optics. The book features among others a chapter on aerosol remote sensing that helps readers to define and solve various aerosol remote sensing problems.
Author | : Miroslaw Jonasz |
Publisher | : Elsevier |
Total Pages | : 715 |
Release | : 2011-08-29 |
Genre | : Science |
ISBN | : 0080548679 |
Light scattering-based methods are used to characterize small particles suspended in water in a wide range of disciplines ranging from oceanography, through medicine, to industry. The scope and accuracy of these methods steadily increases with the progress in light scattering research. This book focuses on the theoretical and experimental foundations of the study and modeling of light scattering by particles in water and critically evaluates the key constraints of light scattering models. It begins with a brief review of the relevant theoretical fundamentals of the interaction of light with condensed matter, followed by an extended discussion of the basic optical properties of pure water and seawater and the physical principles that explain them. The book continues with a discussion of key optical features of the pure water/seawater and the most common components of natural waters. In order to clarify and put in focus some of the basic physical principles and most important features of the experimental data on light scattering by particles in water, the authors employ simple models. The book concludes with extensive critical reviews of the experimental constraints of light scattering models: results of measurements of light scattering and of the key properties of the particles: size distribution, refractive index (composition), structure, and shape. These reviews guide the reader through literature scattered among more than 210 scientific journals and periodicals which represent a wide range of disciplines. A special emphasis is put on the methods of measuring both light scattering and the relevant properties of the particles, because principles of these methods may affect interpretation and applicability of the results. The book includes extensive guides to literature on light scattering data and instrumentation design, as well as on the data for size distributions, refractive indices, and shapes typical of particles in natural waters. It also features a comprehensive index, numerous cross-references, and a reference list with over 1370 entries. An errata sheet for this work can be found at: http://www.tpdsci.com/Ref/Jonasz_M_2007_LightScatE.php *Extensive reference section provides handy compilations of knowledge on the designs of light scattering meters, sources of experimental data, and more *Worked exercises and examples throughout
Author | : Charles Lewis Goolsby |
Publisher | : |
Total Pages | : 270 |
Release | : 1985 |
Genre | : |
ISBN | : |
Author | : Tobili Y. Sam-Yellowe |
Publisher | : Springer Nature |
Total Pages | : 461 |
Release | : 2021-08-01 |
Genre | : Medical |
ISBN | : 3030646866 |
A two-in-one text providing teaching lab students with an overview of immunology as well as a lab manual complete with current standard exercises. Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. Section II consists of twenty-two lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions.