The Institute of Electrical and Electronics Engineers (IEEE) 802.15.4 standard presents a very useful technology for implementing low-cost, low-power, wireless sensor networks. Its main focus, which is to applications requiring simple wireless connectivity with relaxed throughout and latency requirements, makes it suitable for connecting devices that have not been networked, such as industrial and control instrumentation equipments, agricultural equipments, vehicular equipments, and home appliances. Its usage of the license-free 2.4 GHz frequency band makes the technique successful for fast and worldwide market deployments. However, concerns about interference have arisen due to the presence of other wireless technologies using the same spectrum. Although the IEEE 802.15.4 standard has provided some mechanisms, to enhance capability to coexist with other wireless devices operating on the same frequency band, including Carrier Sensor Multiple Access (CSMA), Clear Channel Assessment (CCA), channel alignment, and low duty cycle, it is essential to design and implement adjustable mechanisms for an IEEE 802.15.4 based system integrated into a practical application to deal with interference which changes randomly over time. Among the potential interfering systems (Wi-Fi, Bluetooth, cordless phones, microwave ovens, wireless headsets, etc) which work on the same Industrial, Scientific, and Medical (ISM) frequency band, Wi-Fi systems (IEEE 802.11 technique) have attracted most concerns because of their high transmission power and large deployment in both residential and office environments. This thesis aims to propose a methodology for IEEE 802.15.4 wireless systems to adopt proper adjustment in order to mitigate the effect of interference caused by IEEE 802.11 systems through energy detection, channel agility and data recovery. The contribution of this thesis consists of five parts. Firstly, a strategy is proposed to enable IEEE 802.15.4 systems to maintain normal communications using the means of consecutive transmissions, when the system s default mechanism of retransmission is insufficient to ensure successful rate due to the occurrence of Wi-Fi interference. Secondly, a novel strategy is proposed to use a feasible way for IEEE 802.15.4 systems to estimate the interference pattern, and accordingly adjust system parameters for the purpose of achieving optimized communication effectiveness during time of interference without relying on hardware changes and IEEE 802.15.4 protocol modifications. Thirdly, a data recovery mechanism is proposed for transport control to be applied for recovering lost data by associating with the proposed strategies to ensure the data integrity when IEEE 802.15.4 systems are suffering from interference. Fourthly, a practical case is studied to discuss how to design a sustainable system for home automation application constructed on the basis of IEEE 802.15.4 technique. Finally, a comprehensive design is proposed to enable the implementation of an interference mitigation strategy for IEEE 802.15.4 based ad hoc WSNs within a structure of building fire safety monitoring system. The proposed strategies and system designs are demonstrated mainly through theoretical analysis and experimental tests. The results obtained from the experimental tests have verified that the interference caused by an IEEE 802.11 system on an IEEE 802.15.4 system can be effectively mitigated through adjusting IEEE 802.15.4 system s parameters cooperating with interference pattern estimation. The proposed methods are suitable to be integrated into a system-level solution for an IEEE 802.15.4 system to deal with interference, which is also applicable to those wireless systems facing similar interference issues to enable the development of efficient mitigation strategies.