Gene Cloning And Manipulation
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Author | : Christopher Howe |
Publisher | : Cambridge University Press |
Total Pages | : 250 |
Release | : 2007-07-12 |
Genre | : Medical |
ISBN | : 1139463586 |
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Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.
Author | : C. J. Howe |
Publisher | : Cambridge University Press |
Total Pages | : 228 |
Release | : 1995-03-31 |
Genre | : Medical |
ISBN | : 9780521407007 |
Download Gene Cloning and Manipulation Book in PDF, Epub and Kindle
"Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduate, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations."--BOOK JACKET.
Author | : R. W. Old |
Publisher | : Univ of California Press |
Total Pages | : 228 |
Release | : 1981 |
Genre | : Science |
ISBN | : 9780520046269 |
Download Principles of Gene Manipulation Book in PDF, Epub and Kindle
Author | : Professor of Economics School of Oriental and African Studies Christopher Howe |
Publisher | : |
Total Pages | : 278 |
Release | : 2014-05-14 |
Genre | : Molecular cloning |
ISBN | : 9780511296536 |
Download Gene Cloning and Manipulation Book in PDF, Epub and Kindle
Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.
Author | : David M. Glover |
Publisher | : Springer |
Total Pages | : 231 |
Release | : 2013-12-19 |
Genre | : Medical |
ISBN | : 1489932461 |
Download Gene Cloning Book in PDF, Epub and Kindle
This book was originallyconceived in the form ofa second edition ofa volume published in 1980 in Chapman and Hall's 'OutllneStudies in Biology' series and entitled Genetic Engineering - Cloning DNA. It very rapidly became apparent that with the impact ofrecombinant DNA techniques being feIt in so many areas ofblology, it was going to be difficultifnotimpossible to keepthe bookwithin the space confines of these little monographs. The stays were therefore loosened and the book expanded comfortably to its present size. I hope that this extra space has allowed me to clarify sections ofthe text that were 'heavy going' in the earlierversion. Theextraspace has certainlyallowed me to cover topics that were not mentioned at all in the earlier book. These are primarily to be found in Chapters 7 and 8, which cover the rapid advances that have been recently made in the use ofplantand animal cells as hosts for recombinant DNAmolecules. The develop ment ofother vectors has certainly not stood still over the past four years. This has necessitated a thorough revision ofChapters 3 and 4, which deal with bacteriophage and bacterial plasmid vectors. Numerous techniques for in vitromutagenesis have now been tried and tested allowing me to givecomprehensive coverage ofthisarea in Chapter 2 along with the biochemical techniques used to construct recombinant DNA molecules. Readers with some background knowledge of the approaches to gene cloning will be able to go straight toapart ofthe book in whichthey are specificallyinterested.
Author | : Kakoli Bose |
Publisher | : Springer Nature |
Total Pages | : 315 |
Release | : 2022-01-25 |
Genre | : Medical |
ISBN | : 9811649871 |
Download Textbook on Cloning, Expression and Purification of Recombinant Proteins Book in PDF, Epub and Kindle
This book is immensely useful for graduate students as well as researchers to understand the basics of molecular biology and Recombinant DNA Technology. It provides a comprehensive overview of different approaches for the synthesis of recombinant proteins from E. coli including their cloning, expression and purification. Recent advances in genomics, proteomics, and bioinformatics have facilitated the use of Recombinant DNA Technology for evaluating the biophysical and biochemical properties of various proteins. The book starts with an introductory chapter on gene cloning, protein expression and purification and its implication in current research and commercial applications. Each chapter provides a lucid set of principles, tools and techniques for both students and instructors. The protocols described have been aptly exemplified, and troubleshooting techniques have been included to aid better understanding. Moreover, the set of questions at the end of each chapter have been particularly formulated to help effective learning.
Author | : National Research Council |
Publisher | : National Academies Press |
Total Pages | : 254 |
Release | : 2004-07-08 |
Genre | : Science |
ISBN | : 0309166152 |
Download Safety of Genetically Engineered Foods Book in PDF, Epub and Kindle
Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
Author | : R. W. Old |
Publisher | : Univ of California Press |
Total Pages | : 156 |
Release | : 1980 |
Genre | : Science |
ISBN | : 9780632006274 |
Download Principles of Gene Manipulation Book in PDF, Epub and Kindle
Author | : Dominic Wong |
Publisher | : Springer Science & Business Media |
Total Pages | : 227 |
Release | : 2007-12-31 |
Genre | : Science |
ISBN | : 0387286799 |
Download The ABCs of Gene Cloning Book in PDF, Epub and Kindle
Clear and concise, this easy-to-use text offers an introductory course on the language of gene cloning, covering microbial, plant, and animal systems. The essential concepts in biology relevant to the understanding of gene cloning are presented in a well-organized and accessible manner. This updated version of the first edition is an invaluable book for nonscientists as well as scientists with little background knowledge in gene cloning, providing a wealth of information for anyone wishing to gain proficiency in reading and speaking the language of gene cloning.
Author | : Sue Carson |
Publisher | : Elsevier |
Total Pages | : 172 |
Release | : 2005-12-15 |
Genre | : Science |
ISBN | : 0080456545 |
Download Manipulation and Expression of Recombinant DNA Book in PDF, Epub and Kindle
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein—students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs*Student-tested labs proven successful in a real classroom laboratories*Exercises simulate a cloning project that would be performed in a real research lab*"Project" approach to experiments gives students an overview of the entire process*Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions