Fluorescence Microscopy In Life Sciences
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Author | : Juan Carlos Stockert |
Publisher | : Bentham Science Publishers |
Total Pages | : 751 |
Release | : 2017-12-15 |
Genre | : Science |
ISBN | : 1681085186 |
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Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.
Author | : Juan Carlos Stockert |
Publisher | : |
Total Pages | : |
Release | : 2017-12-15 |
Genre | : |
ISBN | : 9781681085197 |
Download Fluorescence Microscopy in Life Sciences Book in PDF, Epub and Kindle
Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained. Key learning points covered in the book include: Operation of fluorescence microscopy instruments as well as the different options available today for the scientist, from the classical to the most recent approaches The wide range of biological detection possibilities that fluorescence microscopy offers in molecular biology, cell biology, histology and histopathology Fluorescent chemical compounds Breakthroughs in this field, such as non-linear microscopies and super-resolution techniques Fluorescence Microscopy In Life Sciences is intended as a detailed guide for professionals, researchers and students (including graduates Ph.D. candidates) in life sciences, with special emphasis in the biomedical field. Researchers working in allied disciplines such as pharmacology, veterinary sciences and microbiology will also benefit from the information presented in this handbook.
Author | : Anda Cornea |
Publisher | : Elsevier |
Total Pages | : 261 |
Release | : 2014-02-24 |
Genre | : Science |
ISBN | : 0124167136 |
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Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions
Author | : Ulrich Kubitscheck |
Publisher | : John Wiley & Sons |
Total Pages | : 508 |
Release | : 2017-03-27 |
Genre | : Medical |
ISBN | : 3527687726 |
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Zu dem Thema gibt es viele Publikationen, die von Experten für Experten geschrieben wurden. Dieses Buch wendet sich insbesondere an Studenten höherer Semester und Forscher, denen das Hintergrundwissen der Physik fehlt, um neuartige Verfahren der Fluoreszenzmikroskopie zu verstehen. Die zweite Auflage wartet mit neuen Kapiteln und einer erweiterten Einführung auf. Der Schwerpunkt liegt auf der hochauflösenden und Einzelmolekül-Mikroskopie. Jedes Kapitel wurde von einem anerkannten Experten des Fachgebiets geschrieben und sorgfältig überarbeitet, um so die Entwicklungen der letzten Jahre wiederzugeben.
Author | : Jan Toporski |
Publisher | : Springer |
Total Pages | : 603 |
Release | : 2018-03-01 |
Genre | : Science |
ISBN | : 3319753800 |
Download Confocal Raman Microscopy Book in PDF, Epub and Kindle
This second edition provides a cutting-edge overview of physical, technical and scientific aspects related to the widely used analytical method of confocal Raman microscopy. The book includes expanded background information and adds insights into how confocal Raman microscopy, especially 3D Raman imaging, can be integrated with other methods to produce a variety of correlative microscopy combinations. The benefits are then demonstrated and supported by numerous examples from the fields of materials science, 2D materials, the life sciences, pharmaceutical research and development, as well as the geosciences.
Author | : Alberto Diaspro |
Publisher | : Springer Science & Business Media |
Total Pages | : 252 |
Release | : 2010-11-25 |
Genre | : Science |
ISBN | : 3642151752 |
Download Optical Fluorescence Microscopy Book in PDF, Epub and Kindle
In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.
Author | : Ewa M. Goldys |
Publisher | : John Wiley & Sons |
Total Pages | : 398 |
Release | : 2009-08-24 |
Genre | : Science |
ISBN | : 0470083700 |
Download Fluorescence Applications in Biotechnology and Life Sciences Book in PDF, Epub and Kindle
A self-contained treatment of the latest fluorescence applications in biotechnology and the life sciences This book focuses specifically on the present applications of fluorescence in molecular and cellular dynamics, biological/medical imaging, proteomics, genomics, and flow cytometry. It raises awareness of the latest scientific approaches and technologies that may help resolve problems relevant for the industry and the community in areas such as public health, food safety, and environmental monitoring. Following an introductory chapter on the basics of fluorescence, the book covers: labeling of cells with fluorescent dyes; genetically encoded fluorescent proteins; nanoparticle fluorescence probes; quantitative analysis of fluorescent images; spectral imaging and unmixing; correlation of light with electron microscopy; fluorescence resonance energy transfer and applications; monitoring molecular dynamics in live cells using fluorescence photo-bleaching; time-resolved fluorescence in microscopy; fluorescence correlation spectroscopy; flow cytometry; fluorescence in diagnostic imaging; fluorescence in clinical diagnoses; immunochemical detection of analytes by using fluorescence; membrane organization; and probing the kinetics of ion pumps via voltage-sensitive fluorescent dyes. With its multidisciplinary approach and excellent balance of research and diagnostic topics, this book is an essential resource for postgraduate students and a broad range of scientists and researchers in biology, physics, chemistry, biotechnology, bioengineering, and medicine.
Author | : Partha Pratim Mondal |
Publisher | : Springer Science & Business Media |
Total Pages | : 219 |
Release | : 2013-12-12 |
Genre | : Science |
ISBN | : 9400775458 |
Download Fundamentals of Fluorescence Microscopy Book in PDF, Epub and Kindle
This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
Author | : F. W. D. Rost |
Publisher | : Cambridge University Press |
Total Pages | : 486 |
Release | : 1992 |
Genre | : Science |
ISBN | : 9780521410885 |
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Fluorescence microscopy is used for studying the distribution of substances which are present in very small amounts, for example in living cells. This magnificent new work provides comprehensive cover of all aspects of fluorescence microscopy - including instrumentation, applications, and the history of the technique. The first volume deals with instrumentation and techniques for fluorescence microscopy, and includes a chapter on quantification and scanning. The second volume deals with the applications of fluorescence microscopy in many fields. It includes information on autofluorescence, and an invaluable appendix provides an alphabetical list of fluorochromes, giving information concerning chemical structure, fluorescence properties, applications and suitable filter combinations.
Author | : |
Publisher | : Academic Press |
Total Pages | : 523 |
Release | : 1989-04-01 |
Genre | : Science |
ISBN | : 0080859283 |
Download Fluorescence Microscopy of Living Cells in Culture, Part B Book in PDF, Epub and Kindle
Fluorescence Microscopy of Living Cells in Culture, Part B