Dengue Fever is the fastest emerging arboviral infection with major public health consequences for millions of people around the world, and in particular the South-East Asia region. These regional guidelines were extensively revised, updated and expanded with the focus on new and additional topics of current relevance to the populations of the region. They are intended to provide guidance to national and local-level program managers and public health officials, as well as stakeholders, including health practitioners, laboratory personnel and multisectoral partners, in strategic planning, implementation, monitoring and evaluation, and strengthening the response to dengue prevention and control in their countries. Scientists and researchers involved in vaccine and antiviral drug development will also find crucial baseline information in this publication.