Engineering The Genetic Code And Expanding The Genetic Code For In Vivo Incorporation Of Noncanonical Amino Acids Into Biologically Active Ribosomal Peptides
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Author | : |
Publisher | : |
Total Pages | : 95 |
Release | : 2015 |
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Download Engineering the Genetic Code and Expanding the Genetic Code for in Vivo Incorporation of Noncanonical Amino Acids Into Biologically Active Ribosomal Peptides Book in PDF, Epub and Kindle
Author | : |
Publisher | : Academic Press |
Total Pages | : 334 |
Release | : 2009-07-24 |
Genre | : Science |
ISBN | : 0080921639 |
Download Non-Natural Amino Acids Book in PDF, Epub and Kindle
By combining the tools of organic chemistry with those of physical biochemistry and cell biology, Non-Natural Amino Acids aims to provide fundamental insights into how proteins work within the context of complex biological systems of biomedical interest. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. With more than 400 volumes published, each Methods in Enzymology volume presents material that is relevant in today's labs -- truly an essential publication for researchers in all fields of life sciences. Demonstrates how the tools and principles of chemistry combined with the molecules and processes of living cells can be combined to create molecules with new properties and functions found neither in nature nor in the test tube Presents new insights into the molecular mechanisms of complex biological and chemical systems that can be gained by studying the structure and function of non-natural molecules Provides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods
Author | : Jesko Koehnke |
Publisher | : Royal Society of Chemistry |
Total Pages | : 392 |
Release | : 2017-12-15 |
Genre | : Science |
ISBN | : 1782625283 |
Download Cyclic Peptides Book in PDF, Epub and Kindle
This book provides the reader with a comprehensive view of the state-of-the-art of cyclic peptides, from construction to utility in biology and drug discovery.
Author | : Jeffrey Kunio Takimoto |
Publisher | : |
Total Pages | : 150 |
Release | : 2011 |
Genre | : |
ISBN | : 9781124803968 |
Download Unnatural Amino Acid Incorporation for Genetic Code Expansion in Mammalian Cells Book in PDF, Epub and Kindle
The genetic code of most organisms was evolved to encode 20 amino acids. Although the ability to encode 20 amino acids provides the basis to translate proteins necessary for life, researchers are also limited to these 20 amino acids for conventional site-directed mutagenesis. The ability to encode unnatural amino acids provides researchers the ability to circumvent the limitation imposed by the genetic code. Genetically encoding unnatural amino acids provides researchers the means to not only mimic naturally occurring posttranslational modifications but also the ability to encode amino acids with new physical or chemical properties to study biological processes. The incorporation of unnatural amino acids into proteins had been developed in Escherichia coli and also in yeast. We have developed a methodology to genetically incorporate unnatural amino acids in mammalian cells in response to an amber codon (UAG). The incorporation of unnatural amino acids is high in E. coli and yeast, but the incorporation in mammalian cells is relatively low. In addition to developing the system to incorporate unnatural amino acids in mammalian cells, we have also improved suppression efficiencies by modifying the synthetase and unnatural amino acid. To incorporate unnatural amino acids in response to an amber codon, the tRNA anticodon is mutated from a GUA to a CUA. We were able to show that engineering the anticodon-binding domain of the synthetase could enhance the recognition of the tRNA and thus increased suppression efficiencies. Furthermore, by masking the carboxyl group of the amino acid by an ester group, we were able to increase the bioavailability of an unnatural amino acid to further increase suppression efficiencies. Most evolved synthetases aminoacylate unnatural amino acids that are structurally similar to the native substrate of the wild-type synthetase. We were able to adapt Methanosarcina mazei pyrrolysine synthetase (PylRS) to charge a considerable disparate amino acid from its native substrate, O-methyl-L-tyrosine. In addition, the X-ray crystal structure was solved for the evolved PylRS complexed with O-methyl-L-tyrosine at 1.75Å. This multifaceted approach provides the basis to engineer the PylRS to incorporate a significantly diverse selection of unnatural amino acids than previously anticipated.
Author | : Nediljko Budisa |
Publisher | : John Wiley & Sons |
Total Pages | : 312 |
Release | : 2006-05-12 |
Genre | : Science |
ISBN | : 3527607099 |
Download Engineering the Genetic Code Book in PDF, Epub and Kindle
The ability to introduce non-canonical amino acids in vivo has greatly expanded the repertoire of accessible proteins for basic research and biotechnological application. Here, the different methods and strategies to incorporate new or modified amino acids are explained in detail, including a lot of practical advice for first-time users of this powerful technique. Novel applications in protein biochemistry, genomics, biotechnology and biomedicine made possible by the expansion of the genetic code are discussed and numerous examples are given. Essential reading for all molecular life scientists who want to stay ahead in their research.
Author | : Anton Glieder |
Publisher | : Springer |
Total Pages | : 371 |
Release | : 2015-11-03 |
Genre | : Science |
ISBN | : 3319227084 |
Download Synthetic Biology Book in PDF, Epub and Kindle
This book provides a comprehensive, up-to-date overview of the opportunities and challenges of the complex field of synthetic biology, which combines various scientific disciplines. The emerging field of synthetic biology employs biotechnological approaches to recreate and enhance basic biological structures, intracellular processes and whole organisms. The book addresses a broad range of topics, including redesigning complex metabolic pathways, DNA/RNA and protein engineering, as well as novel synthetic biomaterials. It discusses both “bottom up” and “top down” approaches and presents the latest genome engineering tools with predictions about how these could change our way of thinking and working. Since the use of synthetic biology raises a number of ethical questions, a chapter is devoted to public awareness and risk management.The book is of interest to scientists from both academia and industry, as well as PhD students and postdocs working in the field
Author | : Shōzō Ōsawa |
Publisher | : |
Total Pages | : 240 |
Release | : 1995 |
Genre | : Science |
ISBN | : |
Download Evolution of the Genetic Code Book in PDF, Epub and Kindle
The genetic code was deciphered experimentally around 1966 and for a number of years scientists considered it to be "universal" for all forms of life. In 1981 researchers shocked the scientific community with the discovery that the code differed in mitochondria and certain other organisms, evidence that the genetic code was still evolving. This book discusses the distribution and origin of the non-universal codes and examines the possible mechanisms of code changes, making it essential reading for all those interested in evolutionary genetics.
Author | : David A Liberles |
Publisher | : Oxford University Press |
Total Pages | : 267 |
Release | : 2007-05-31 |
Genre | : Science |
ISBN | : 0199299188 |
Download Ancestral Sequence Reconstruction Book in PDF, Epub and Kindle
Ancestral sequence reconstruction is a technique of growing importance in molecular evolutionary biology and comparative genomics. As a powerful tool for testing evolutionary and ecological hypotheses, as well as uncovering the link between sequence and molecular phenotype, there are potential applications in a range of fields.Ancestral Sequence Reconstruction starts with a historical overview of the field, before discussing the potential applications in drug discovery and the pharmaceutical industry. This is followed by a section on computational methodology, which provides a detailed discussion of the available methods for reconstructing ancestral sequences (including their advantages, disadvantages, and potential pitfalls). Purely computational applications of the technique are then covered, including wholeproteome reconstruction. Further chapters provide a detailed discussion on taking computationally reconstructed sequences and synthesizing them in the laboratory. The book concludes with a description of the scientific questions where experimental ancestral sequence reconstruction has been utilized toprovide insights and inform future research.This research level text provides a first synthesis of the theories, methodologies and applications associated with ancestral sequence recognition, while simultaneously addressing many of the hot topics in the field. It will be of interest and use to both graduate students and researchers in the fields of molecular biology, molecular evolution, and evolutionary bioinformatics.
Author | : |
Publisher | : Elsevier |
Total Pages | : 4266 |
Release | : 2020-07-22 |
Genre | : Science |
ISBN | : 0081026919 |
Download Comprehensive Natural Products III Book in PDF, Epub and Kindle
Comprehensive Natural Products III, Third Edition, Seven Volume Set updates and complements the previous two editions, including recent advances in cofactor chemistry, structural diversity of natural products and secondary metabolites, enzymes and enzyme mechanisms and new bioinformatics tools. Natural products research is a dynamic discipline at the intersection of chemistry and biology concerned with isolation, identification, structure elucidation, and chemical characteristics of naturally occurring compounds such as pheromones, carbohydrates, nucleic acids and enzymes. This book reviews the accumulated efforts of chemical and biological research to understand living organisms and their distinctive effects on health and medicine and to stimulate new ideas among the established natural products community. Provides readers with an in-depth review of current natural products research and a critical insight into the future direction of the field Bridges the gap in knowledge by covering developments in the field since the second edition published in 2010 Split into 7 sections on key topics to allow students, researchers and professionals to find relevant information quickly and easily Ensures that the knowledge within is easily understood by and applicable to a large audience
Author | : |
Publisher | : Academic Press |
Total Pages | : 494 |
Release | : 2001-03-21 |
Genre | : Science |
ISBN | : 9780121822323 |
Download Hyperthermophilic Enzymes Book in PDF, Epub and Kindle
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymologyis one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. This volume and its companions (Volumes 330 and 334) cover all current knowledge concerning hyperthermophilic enzymes. Major topics in this volume include the enzymes of central metabolism and respiratory enzymes.