Growth of Vesicular Stomatitis Virus in Mosquito Cell Lines [Microfilm]

Growth of Vesicular Stomatitis Virus in Mosquito Cell Lines [Microfilm]
Author: Harvey Artsob
Publisher:
Total Pages: 0
Release: 1973
Genre: Stomatitis in animals
ISBN:

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"Cocal strain of vesicular stomatitis virus was propagated in Aedes albopictus and Aedes aegypti cell lines. Infection of the cell lines at 30°C resulted in a low titered virus infection involving only a small proportion of the cell population with no cytopathic effects. Virus titers in A. albopictus were increased by adsorption with DEAE dextran or by increasing temperature of incubation. Use of a high input multiplicity and incubation of cells at 37°C resulted in 100% infection of cell cultures allowing a limited electron microscopic study of virus development. Persistent infection was established in both mosquito cell lines and maintained for up to 60 passages with some change in properties of passaged virus. Characterization of carrier cultures indicated no role for antiviral factors in controlling virus infection, but most cells appeared to be genetically resistant. Treatment of persistently infected A. albopictus with actinomycin D resulted in elevated virus titers." --

Drosophila Cells in Culture

Drosophila Cells in Culture
Author: Guy Echalier
Publisher: Academic Press
Total Pages: 259
Release: 2017-11-30
Genre: Science
ISBN: 0128097043

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Drosophila Cells in Culture, Second Edition, includes comprehensive coverage of cell lines, methods for creating cell lines, methods for genome engineering, and the use of cell lines for genome wide rNAi screens. This publication summarizes over thirty years of experience in the handling of in vitro cultured Drosophila cells alongside recent methods and functional screens. Early and experienced researchers studying drosophila in developmental biology, genetics, neuroscience, and across the biological and biomedical sciences will benefit from this expert knowledge. Offers full coverage of cell lines and primary cultures Provides a go-to resource for methods and studies completed with drosophila cells in culture Presents a wide spectrum of experimental techniques

Drosophila Cells in Culture

Drosophila Cells in Culture
Author: Guy Echalier
Publisher: Academic Press
Total Pages: 735
Release: 1997-02-24
Genre: Science
ISBN: 0080530532

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Currently Drosophila is a dominant experimental model in developmental biology and in gene regulation in eukaryotes. This volume summarizes some thirty years of experience in the handling of in vitro cultured Drosophila cells. Its main emphasis is on gene transfer methodology, cell responses to heat shock, hormonal regulation of genes, and on the expression and mobility of transposable elements. Some thirty years of experience in handling in vitro cultured Drosophila cells Cell cultures which provide material for a multiplicity of biochemical approaches DNA-mediated gene transfer as an irreplaceable tool for analyzing basic mechanisms of regulation Drosophila cell lines which qualify them for use in biotechnology

Origin and Evolution of Viruses

Origin and Evolution of Viruses
Author: Esteban Domingo
Publisher: Elsevier
Total Pages: 573
Release: 2008-06-23
Genre: Science
ISBN: 0080564968

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New viral diseases are emerging continuously. Viruses adapt to new environments at astounding rates. Genetic variability of viruses jeopardizes vaccine efficacy. For many viruses mutants resistant to antiviral agents or host immune responses arise readily, for example, with HIV and influenza. These variations are all of utmost importance for human and animal health as they have prevented us from controlling these epidemic pathogens. This book focuses on the mechanisms that viruses use to evolve, survive and cause disease in their hosts. Covering human, animal, plant and bacterial viruses, it provides both the basic foundations for the evolutionary dynamics of viruses and specific examples of emerging diseases. NEW - methods to establish relationships among viruses and the mechanisms that affect virus evolution UNIQUE - combines theoretical concepts in evolution with detailed analyses of the evolution of important virus groups SPECIFIC - Bacterial, plant, animal and human viruses are compared regarding their interation with their hosts