DNA Damage Detection In Situ, Ex Vivo, and In Vivo

DNA Damage Detection In Situ, Ex Vivo, and In Vivo
Author: Vladimir V. Didenko
Publisher: Humana Press
Total Pages: 0
Release: 2010-11-08
Genre: Medical
ISBN: 9781603274081
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Recent advances in organic chemistry, fluorescent microscopy, and materials science have created an entirely new range of techniques and probes for imaging DNA damage in molecular and cellular biology. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore the latest advances in the area, covering both recent and established techniques to detect and quantify DNA damage at scales ranging from subcellular to the level of a whole live organism. Chapters present all major assays used in molecular and cellular biology for the labeling of DNA damage in situ, ex vivo, and in vivo. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols is an essential handbook for novice and experienced researchers in a variety of fields, including molecular and cellular biology, experimental and clinical pathology, toxicology, radiobiology, oncology, embryology, experimental pharmacology, drug design, and environmental science.

In Situ Detection of DNA Damage

In Situ Detection of DNA Damage
Author: Vladimir V. Didenko
Publisher: Springer Science & Business Media
Total Pages: 314
Release: 2008-02-05
Genre: Medical
ISBN: 1592591795
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Detection and analysis of DNA damage is of critical importance in a variety of biological disciplines studying apoptosis, cell cycle and cell di- sion, carcinogenesis, tumor growth, embryogenesis and aging, neu- degenerative and heart diseases, anticancer drug development, environmental and radiobiological research, and others. Individual cells within the same tissue or in cell culture may vary in the extent of their DNA damage and, consequently, can display different re- tions to it. These differences between individual cells in the same cell popu- tion are detected using in situ approaches. In situ is a Latin term meaning “on site” or “in place.” It is used to denote the processes occurring or detected in their place of origin. In mole- lar and cell biology this usually refers to undisrupted mounted cells or tissue sections. In that meaning “in situ” is used as part of the terms “in situ PCR,” “in situ transcription,” “in situ hybridization,” “in situ end labeling,” and “in situ ligation.” Sometimes the “in situ” term is applied at the subcellular level to cells disrupted in the process of analysis, for example, in the detection of specific sequences in chromosomes using fluorescent in situ hybridization (FISH). Historically, the term was used primarily in methods dealing with nucleic acids.

DNA Damage and Repair in Human Tissues

DNA Damage and Repair in Human Tissues
Author: Betsy M. Sutherland
Publisher: Springer Science & Business Media
Total Pages: 456
Release: 2012-12-06
Genre: Medical
ISBN: 146130637X
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Physical and chemical agents in the environment damage the DNA of humans, and pose a major threat to human health today, and to the genetic integrity of human populations. Although studies on isolated DNA in vitro, on prokaryotes, on mammalian cells in culture, and on laboratory animals have provided essential background information, it is now possible to study DNA damage and repair in human tissues directly. New techniques of high sensitivity, especially those not requiring radioactive labeling have made possible quantitation of DNA damage and repair, as well as detection of residual, unrepaired DNA lesions . In recent years, several investigators have taken up the challenge of studying damage and repair responses in humans, and we have chosen that work as the special focus of this Symposium. Major advances in under standing damage and responses in human skin, in blood cells and in human internal organs indicate three major themes. First, DNA damage levels in human tissues depend not only on the initial exposures, but also on the capapacity of that tissue for repair of the specific lesion type. Second, repair in human tissues may differ quantitatively and qualitatively from that in human cells in culture.

On-Treatment DNA Damage Response Biomarkers in Surrogate Tissue Types

On-Treatment DNA Damage Response Biomarkers in Surrogate Tissue Types
Author: Katherine McDermott
Publisher:
Total Pages:
Release: 2016
Genre:
ISBN:
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In order to study these specimens, consent was obtained from healthy volunteers for the collection of hair follicles and whole blood, treated ex vivo, and evaluated by immunohistochemistry and flow cytometry for biomarkers of DNA damage. As an expansion of the whole blood work, blood was also obtained from rats after in vivo exposure to various DNA damaging agents.

Recent Advances in Cytometry

Recent Advances in Cytometry
Author: Zbigniew Darzynkiewicz
Publisher: Academic Press
Total Pages: 617
Release: 2011
Genre: Cell Separation
ISBN: 0123749123
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Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay. Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays * Discusses many new assays developed since the previous edition. Presents recent developments in cytometric intrumentation/technology.

Basic Clinical Radiobiology

Basic Clinical Radiobiology
Author: Michael C. Joiner
Publisher: CRC Press
Total Pages: 711
Release: 2018-08-28
Genre: Medical
ISBN: 0429955391
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Basic Clinical Radiobiology is a concise but comprehensive textbook setting out the essentials of the science and clinical application of radiobiology for those seeking accreditation in radiation oncology, clinical radiation physics, and radiation technology. Fully revised and updated to keep abreast of current developments in radiation biology and radiation oncology, this fifth edition continues to present in an interesting way the biological basis of radiation therapy, discussing the basic principles and significant developments that underlie the latest attempts to improve the radiotherapeutic management of cancer. This new edition is highly illustrated with attractive 2-colour presentation and now includes new chapters on stem cells, tissue response and the convergence of radiotherapy, radiobiology, and physics. It will be invaluable for FRCR (clinical oncology) and equivalent candidates, SpRs (and equivalent) in radiation oncology, practicing radiation oncologists and radiotherapists, as well as radiobiologists and radiotherapy physicists.

Toxicity of Nanoparticles

Toxicity of Nanoparticles
Author: Mohammed Muzibur Rahman
Publisher: BoD – Books on Demand
Total Pages: 256
Release: 2024-01-10
Genre: Technology & Engineering
ISBN: 1837684529
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Toxicity of Nanoparticles - Recent Advances and New Perspectives covers all the important nanotechnological aspects of various metallic nanoparticles, including their growth and characterization, facile synthesis and fabrication, development, and potential applications for industrial, environmental, and healthcare purposes. Recent developments, innovative synthesis methods, metal oxides, drug delivery, bioengineering, microbiology, seafood and aquacultures, phytometallics, nanotoxicological assessment, immunotoxicity, and promising new frontiers around these advanced nanoscale materials are reviewed under the editorship of an internationally renowned scientist. This book is useful for a diverse range of readers and important for research organizations, government research centers, academic libraries, and those involved in the research and development of carbon materials.

Recent Progress in Pharmaceutical Nanobiotechnology: A Medical Perspective

Recent Progress in Pharmaceutical Nanobiotechnology: A Medical Perspective
Author: Habibe Yılmaz
Publisher: Bentham Science Publishers
Total Pages: 442
Release: 2023-12-28
Genre: Science
ISBN: 9815179438
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Recent Progress in Pharmaceutical Nanobiotechnology: A Medical Perspective offers a comprehensive exploration of the dynamic field of pharmaceutical nanobiotechnology, focusing on its medical applications. This edited reference serves as a valuable resource for researchers, students, and professionals in various disciplines (pharmacology, biotechnology, clinical medicine and nanotechnology) , providing insights into the latest advancements and practical implications of nanotechnology in the pharmaceutical sector. The book presents 14 edited and referenced chapters that cover several themes for readers. General Pharmaceutical Nanobiotechnology: Introduction to the interdisciplinary field Exploration of nanoscale materials for medical purposes Nanoparticle Development and Applications: Bioinspired Nanomedicines Lipid-Based Nanocarriers Metallic Nanoparticles and Their Applications Nanoparticle Targeting Strategies Nanomedicine-Based Therapies for Cancer Stem Cells Biotechnological Aspects: Biotechnological Significance of Exosomes Glycoconjugates: Biosynthesis and Functions Innovative Nanotherapies: Novel Nanotechnological Approaches for Glioblastoma Biocompatibility of Nanomedicines and Bio Corona Diagnostic and Sensing Applications: Role of Nanoparticular/Nano Vesicular Systems as Biosensors In Vitro Applications of Drug-Carrying Nanoparticles in Cell Culture Studies In Vivo Imaging Techniques: Bioluminescence and Fluorescence Imaging Precision Medicine: The Role of Nano and Biopharmaceutics in Precision Medicine Audience Postgraduate researchers in pharmaceutical biotechnology; pharmacy professionals and academicians