Download Development And Application Of Post Capillary Affinity Detection For Capillary Electrophoresis full books in PDF, epub, and Kindle. Read online free Development And Application Of Post Capillary Affinity Detection For Capillary Electrophoresis ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
| Author | : Jean Abler Kelly |
| Publisher | : |
| Total Pages | : 200 |
| Release | : 1997 |
| Genre | : |
| ISBN | : |
| Author | : Mark A. Strege |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 340 |
| Release | : 2008-02-04 |
| Genre | : Science |
| ISBN | : 159259798X |
Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive. Over time, CE has matured to become a superb complement to HLPC, and in many cases has also evolved as an automated and quantitative replacement for conventional slab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. Within Capillary Electrophoresis of Proteins and Peptides, we have assembled contributions from researchers who are applying state-of-the-art CE for protein and peptide analysis, including topics that we believe are of great potential both in the present and for the future. In comparison to traditional separation methods, CE represents a miniaturized analysis technique (especially in its microchip-based format) that is highly dependent upon the basic fundamentals of effective sample recovery and high sensitivity detection. With these issues in mind, Chapters 1–4 describe recently developed approaches for both capillary coatings and analyte detection via laser-induced fluorescence. Since the discipline of biotechnology has established itself as a primary platform for the application of CE to the analysis of proteins and peptides, Chapters 5–7 demonstrate a variety of examples of the specific techniques that have been applied for the development of biopharmaceuticals and their commercialization. The methods covered here include also the analysis of oligosaccharides from glycoproteins.
| Author | : Sahana Bose |
| Publisher | : |
| Total Pages | : 170 |
| Release | : 1997 |
| Genre | : |
| ISBN | : |
| Author | : Reinhard H. H. Neubert |
| Publisher | : CRC Press |
| Total Pages | : 392 |
| Release | : 2003-01-14 |
| Genre | : Medical |
| ISBN | : 0203912136 |
This reference presents the most recent breakthroughs and techniques in affinity capillary electrophoresis (ACE) to measure and determine the physicochemical and thermodynamic parameters of drug compounds. The authors offer strategies to explore and characterize interactions between drugs, drug vehicles, and biological membranes to facilitate devel
| Author | : Carlos D. García |
| Publisher | : John Wiley & Sons |
| Total Pages | : 638 |
| Release | : 2013-02-01 |
| Genre | : Science |
| ISBN | : 1118529995 |
Explores the benefits and limitations of the latest capillary electrophoresis techniques Capillary electrophoresis and microchip capillary electrophoresis are powerful analytical tools that are particularly suited for separating and analyzing biomolecules. In comparison with traditional analytical techniques, capillary electrophoresis and microchip capillary electrophoresis offer the benefits of speed, small sample and solvent consumption, low cost, and the possibility of miniaturization. With contributions from a team of leading analytical scientists, Capillary Electrophoresis and Microchip Capillary Electrophoresis explains how researchers can take full advantage of all the latest techniques, emphasizing applications in which capillary electrophoresis has proven superiority over other analytical approaches. The authors not only explore the benefits of each technique, but also the limitations, enabling readers to choose the most appropriate technique to analyze a particular sample. The book's twenty-one chapters explore fundamental aspects of electrophoretically driven separations, instrumentation, sampling techniques, separation modes, detection systems, optimization strategies for method development, and applications. Specific topics include: Critical evaluation of the use of surfactants in capillary electrophoresis Sampling and quantitative analysis in capillary electrophoresis Capillary electrophoresis with electrochemical detection Overcoming challenges in using microchip electrophoresis for extended monitoring applications Capillary electrophoresis of intact unfractionated heparin and related impurities Microchip capillary electrophoresis for in situ planetary exploration Each chapter begins with an introduction and ends with conclusions as well as references to the primary literature. Novices to the field will find this book an easy-to-follow introduction to core capillary electrophoresis techniques and methods. More experienced investigators can turn to the book for troubleshooting tips and expert advice to guide them through the most advanced applications.
| Author | : Suvardhan Kanchi |
| Publisher | : CRC Press |
| Total Pages | : 396 |
| Release | : 2017-08-21 |
| Genre | : Medical |
| ISBN | : 1351846736 |
Capillary electrophoresis (CE) has become an established method with widespread recognition as an analytical technique of choice in numerous analytical laboratories, including industrial and academic sectors. Pharmaceutical and biochemical research and quality control are the most important CE applications. This book provides a comparative assessment of related techniques on mode selection, method development, detection, and quantitative analysis and estimation of pharmacokinetic parameters and broadens the understanding of modern CE applications, developments, and prospects. It introduces the fundamentals of CE and clearly outlines the procedures used to mitigate several barriers, such as detection limits, signal detection, changing capillary environment, resolution separation of analytes, and hyphenation of mass spectrometry with CE, for a range of analytical problems. Each chapter outlines a specific electrophoretic variant with detailed instructions and some standard operating procedures. In this respect, the book meets its desired goal of rendering assistance to lovers of electrophoresis.
| Author | : Andras Guttman |
| Publisher | : Newnes |
| Total Pages | : 391 |
| Release | : 2021-12-04 |
| Genre | : Science |
| ISBN | : 0080931359 |
Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses
| Author | : Richard A Frazier |
| Publisher | : Royal Society of Chemistry |
| Total Pages | : 143 |
| Release | : 2007-10-31 |
| Genre | : Technology & Engineering |
| ISBN | : 1847550312 |
Since its inception in the early 1980s, capillary electrophoresis (CE) offers a great deal of flexibility as a modern analytical technique, and has found applications within many fields of analysis, particularly pharmaceutical science and biochemistry. Until now, food analysts have had difficulties in adopting the technique due to the lack of written guidance. Capillary Electrophoresis for Food Analysis: Method Development provides basic information and the support needed to enable food analysts to utilise the technique for the development of new separation methods. Designed specifically for the needs of food analysts, the book takes the reader step by step through the process of developing and troubleshooting CE methods. Worked examples are included to make it ideal as a laboratory companion as well as a library reference source.
| Author | : James P. Landers |
| Publisher | : CRC Press |
| Total Pages | : 918 |
| Release | : 1996-12-23 |
| Genre | : Medical |
| ISBN | : 9780849324987 |
Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science.
| Author | : H. Shintani |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 750 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 9400915616 |
Over the last decade, high performance Capillary electrophoresis (HPCE) has emerged as a powerful and versatile separation technique that promises to rival high performance liquid chromatography when applied to the separation of both charged and neutral species. The high speed and high separation efficiency which can be attained using any of the various modes of HPCE has resulted in the increased use of the technique in a range of analytical environments. The procedures are, however, still in the early stages of development and several barriers remain to their adoption as the technique of choice for a range of analytical problems. One such barrier is the selection and optimization of the conditions required to achieve reproducible separations of analytes and it is in this area that this new book seeks to give assistance. The book is written by an international team of authors, drawn from both academic and industrial users, and the manufacturers of instruments. At its heart are a number of tables, divided into specific application areas. These give details of published separations of a wide range of archetypal analytes, the successful separation conditions and the matrix in which they were presented. These tables are based on separations reported since 1992 and are fully referenced to the original literature. The tables are supported by discussions of the problems that a particular area presents and the strategies and solutions adopted to overcome them. The general areas covered are biochemistry, pharmaceutical science, bioscience, ion analysis, food analysis and environmental science.
