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| Author | : Walter Lowry Caudill |
| Publisher | : |
| Total Pages | : 162 |
| Release | : 1983 |
| Genre | : |
| ISBN | : |
| Author | : Walter Lowry Candill |
| Publisher | : |
| Total Pages | : 398 |
| Release | : 1983 |
| Genre | : Electrochemical apparatus |
| ISBN | : |
| Author | : R. Bruce Holman |
| Publisher | : |
| Total Pages | : 392 |
| Release | : 1993-06-22 |
| Genre | : Medical |
| ISBN | : |
General Editor: A.D. Smith High Performance Liquid Chromatography in Neuroscience Research Edited by R. Bruce Holman, Alan J. Cross and Michael H. Joseph This volume in the Handbook Series begins with a succinct introduction to the principles of High Performance Liquid Chromatography (HPLC) and to the principles of methods development for neuroscience application. The primary emphasis of the book is on detailed and functional description of methods for a wide range of specific groups of neurochemicals, with particular emphasis on the analysis of neurotransmitters and related compounds. The final chapter reviews the application of HPLC to the analysis of central nervous system (CNS)-active drugs. Each chapter is written by experts in their fields. As well as providing a brief overview of the choice of methodologies, they focus on specific methods to provide the maximum amount of practical information based on personal experience, illustrated by material from the analysis of real biological samples.
| Author | : William R. LaCourse |
| Publisher | : Wiley-Interscience |
| Total Pages | : 360 |
| Release | : 1997-08-18 |
| Genre | : Science |
| ISBN | : |
A reliable, comprehensive, relevant view of HPLC and its applications The development of HPLC-PED represents the successful marriage of two powerful analytical technologies and has resulted in the best technique for sensitive and direct detection of biological compounds with poor optical detection properties. PED has been used extensively for the determination of carbohydrates and other polar aliphatic compounds, and as a result, numerous methods have been developed to enable the analysis of a wide variety of samples. Over the years, many articles, anecdotal information, and misinformation have permeated the scientific community, with the possible consequence of confusion or uncertainty with regard to PED on the part of the analyst. Pulsed Electrochemical Detection in High-Performance Liquid Chromatography presents a reliable, comprehensive, and relevant review of HPLC-PED and its applications. The book is divided into three major parts: background material necessary for a more thorough understanding of the principles and relevance of PED; an in-depth discussion of PED using voltammetry and other electroanalytical techniques and presenting the advantages, applicability, and optimization of all existing PED waveforms; and practical aspects of HPLC-PED, including a summary of the major applications and a look at future developments in the technique. Appendices include a pulsed voltammetry (PV) program specifically written to optimize pulsed amperometric detection (PAD) waveforms and all the known applications, categorized and listed in tabular form. For analytical chemists; biochemists; carbohydrate chemists; biotechnologists; undergraduate, graduate, and postdoctoral students; and lab technicians working in a range of areas including the pharmaceutical, medical, and food and beverage industries, this eminently readable guide is the first reliable book-length treatment of how to use PED coupled with HPLC.
| Author | : W.J. Lough |
| Publisher | : CRC Press |
| Total Pages | : 292 |
| Release | : 1995-09-30 |
| Genre | : Science |
| ISBN | : 9780751400762 |
High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.
| Author | : R. Andrew Shalliker |
| Publisher | : CRC Press |
| Total Pages | : 311 |
| Release | : 2011-12-14 |
| Genre | : Science |
| ISBN | : 084939077X |
Widely employed for separating and detecting chemicals in solution, separation techniques are most often applied in tandem, subsequently referred to as hyphenated methods. Hyphenated and Alternative Methods of Detection in Chromatography details the development and application of mass spectral detection techniques coupled with gas phase and liquid phase chromatographies. With contributions from experts in a variety of fields, as evidenced by the range of topics, the book describes the advantages and disadvantages of various separation techniques and addresses methods for cutting-edge applications such as proteomics research. Specifically, this book covers: The development of the LC-NMR and its application in chromatographic science, particularly in relation to the analysis of natural and environmental samples and in the study of reaction monitoring, biosynthetic analysis, and structural elucidation The application of LC-ICP in biological samples for the speciation of organoarsenic and organoselenium compounds; metal complexes in microorganisms, plants, and foods of plant origin; human body fluids and tissues; and in the detection of chemical warfare agents Electrochemical detection in liquid chromatography, system optimization, and applications in the analysis of flavonoids The use of chemiluminescence for microcolumn and capillary methods of separation The process of employing multidimensional separation methods as a means of identifying components in complex samples A detailed reference for those starting out in the field, this book also suits those who are more experienced yet require assistance in new directions. It is an ideal reference for senior undergraduate and postgraduate students wishing to supplement their learning experience. Containing over 800 references, this book is an excellent source of information within the field of hyphenated methods of chromatographic analysis.
| Author | : Csaba Horváth |
| Publisher | : Elsevier |
| Total Pages | : 332 |
| Release | : 2013-10-22 |
| Genre | : Science |
| ISBN | : 1483261883 |
High-Performance Liquid Chromatography: Advances and Perspectives, Volume 4 is an authoritative publication that deals with the fundamentals, instrumentation, and applications of high-performance liquid chromatography. The volume contains articles on practical aspects of reversed-phase chromatography in the study of biopolymer separations; characterization of stationary phases and the development of various packing materials; electrochemical detection; and the fundamentals of chromatographic behavior of large molecules. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book an interesting read.
| Author | : Ian N. Acworth |
| Publisher | : VSP |
| Total Pages | : 396 |
| Release | : 1997 |
| Genre | : Science |
| ISBN | : 9789067642323 |
This sixth volume in the book series Progress in HPLC-HPCE examines the enhancement of high- performance liquid chromatography through the development of an advanced mode of electrochemical detection (ECD) --- the coulometric array detection --- from its initial, yet problematic, amperometric (thin-layer) design to the highly sensitive, selective and stable coulometric (flow-through) design. Unlike amperometric electrodes, the coulometric electrode is 100% efficient and measures signals from all of the analyte passing through it, which leads to improved sensitivity as well as unique selectivity. The coulometric electrode array offers the resolution of the photodiode array with the extreme sensitivity of an electrochemical detector.
| Author | : Robert J Flanagan |
| Publisher | : Royal Society of Chemistry |
| Total Pages | : 244 |
| Release | : 2007-10-31 |
| Genre | : Science |
| ISBN | : 184755072X |
Electrochemical Detection in HPLC: Analysis of Drugs and Poisons is the first monograph devoted to the application of this mode of analysis to the assay of exogenous compounds such as drugs in biological fluids and associated areas. The introductory chapters provide information on basic electrochemistry and HPLC-ED, and on trouble-shooting. The specialized area of thiol analysis is also discussed in detail. Salient practical details of published applications of the technique in analytical toxicology and related areas are provided in a standard format. Alternative techniques are suggested throughout. The emphasis is on the analysis of exogenous compounds, although catecholamines and other endogenous species are discussed in so far as they may be used as drugs. The practical nature of this book will make it useful to professionals working in the field. It will also be of benefit to analysts wishing to use HPLC-ED in the analysis of biological samples for analytes not specifically covered in the volume.
| Author | : Q. Alan Xu |
| Publisher | : John Wiley & Sons |
| Total Pages | : 342 |
| Release | : 2013-04-01 |
| Genre | : Technology & Engineering |
| ISBN | : 1118533984 |
Explores both the benefits and limitations of new UHPLC technology High performance liquid chromatography (HPLC) has been widely used in analytical chemistry and biochemistry to separate, identify, and quantify compounds for decades. The science of liquid chromatography, however, was revolutionized a few years ago with the advent of ultra-high performance liquid chromatography (UHPLC), which made it possible for researchers to analyze sample compounds with greater speed, resolution, and sensitivity. Ultra-High Performance Liquid Chromatography and Its Applications enables readers to maximize the performance of UHPLC as well as develop UHPLC methods tailored to their particular research needs. Readers familiar with HPLC methods will learn how to transfer these methods to a UHPLC platform and vice versa. In addition, the book explores a variety of UHPLC applications designed to support research in such fields as pharmaceuticals, food safety, clinical medicine, and environmental science. The book begins with discussions of UHPLC method development and method transfer between HPLC and UHPLC platforms. It then examines practical aspects of UHPLC. Next, the book covers: Coupling UHPLC with mass spectrometry Potential of shell particles in fast liquid chromatography Determination of abused drugs in human biological matrices Analyses of isoflavones and flavonoids Therapeutic protein characterization Analysis of illicit drugs The final chapter of the book explores the use of UHPLC in drug metabolism and pharmacokinetics studies for traditional Chinese medicine. With its frank discussions of UHPLC's benefits and limitations, Ultra-High Performance Liquid Chromatography and Its Applications equips analytical scientists with the skills and knowledge needed to take full advantage of this new separation technology.
