Biotechnology Applications Of Microinjection Microscopic Imaging And Fluorescence
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| Author | : Peter H. Bach |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 258 |
| Release | : 2012-12-06 |
| Genre | : Medical |
| ISBN | : 1461528283 |
Download Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence Book in PDF, Epub and Kindle
Individual cells behave in surpnsmg ways that cannot be deduced from the averaged results of an organ as assessed by the use of conventional biochemical methods. Thus multicellular plant and animals systems are being investigated by an increasing array of histochemical and cytochemical techniques based on general chemical or specific immunological interactions to identify structural materials and to assess biological activities. In recent years there has been an increasing range of fluorescent probes, along with advanced computerised imaging and analysis techniques, which allows the behaviour of individual living cells to be followed in considerable detail. The parallel use of microinjection, microelectrodes and patch-clamping provides additional information about cells and their responses. Recombinant DNA technology has highlighted the desirability and the power of microinjecting defined materials into specific cells and so manipulating their fundamental biochemistry. New hypotheses are being tested which will form the cornerstone of future developments across the whole spectrum of biotechnology. The First European Workshop on Biotechnology Applications of Microinjection, Microscopic Imaging and Fluorescence was run at the University of East London, U.K, 21st-24th April, 1992 with the objective of bringing together a diverse group of individuals who were using these state-of-the-art applications for biotechnological exploration. A novel feature of the meeting was paiticipation by instrument manufacturers in the programme: there were hands-on workshops (where living cells could be examined), combined with the poster sessions.
| Author | : Jens Rietdorf |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 344 |
| Release | : 2005-06-23 |
| Genre | : Science |
| ISBN | : 9783540236986 |
Download Microscopy Techniques Book in PDF, Epub and Kindle
With contributions by numerous experts
| Author | : Ewa M. Goldys |
| Publisher | : John Wiley & Sons |
| Total Pages | : 398 |
| Release | : 2009-08-24 |
| Genre | : Science |
| ISBN | : 0470083700 |
Download Fluorescence Applications in Biotechnology and Life Sciences Book in PDF, Epub and Kindle
A self-contained treatment of the latest fluorescence applications in biotechnology and the life sciences This book focuses specifically on the present applications of fluorescence in molecular and cellular dynamics, biological/medical imaging, proteomics, genomics, and flow cytometry. It raises awareness of the latest scientific approaches and technologies that may help resolve problems relevant for the industry and the community in areas such as public health, food safety, and environmental monitoring. Following an introductory chapter on the basics of fluorescence, the book covers: labeling of cells with fluorescent dyes; genetically encoded fluorescent proteins; nanoparticle fluorescence probes; quantitative analysis of fluorescent images; spectral imaging and unmixing; correlation of light with electron microscopy; fluorescence resonance energy transfer and applications; monitoring molecular dynamics in live cells using fluorescence photo-bleaching; time-resolved fluorescence in microscopy; fluorescence correlation spectroscopy; flow cytometry; fluorescence in diagnostic imaging; fluorescence in clinical diagnoses; immunochemical detection of analytes by using fluorescence; membrane organization; and probing the kinetics of ion pumps via voltage-sensitive fluorescent dyes. With its multidisciplinary approach and excellent balance of research and diagnostic topics, this book is an essential resource for postgraduate students and a broad range of scientists and researchers in biology, physics, chemistry, biotechnology, bioengineering, and medicine.
| Author | : Karsten König |
| Publisher | : Walter de Gruyter GmbH & Co KG |
| Total Pages | : 785 |
| Release | : 2018-01-22 |
| Genre | : Science |
| ISBN | : 311043007X |
Download Multiphoton Microscopy and Fluorescence Lifetime Imaging Book in PDF, Epub and Kindle
This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue imaging Multiphoton Tomography (MPT) Clinical multimodal CARS imaging In vivo multiphoton microscopy of human skin Two-photon microscopy and fluorescence lifetime imaging of the cornea Multiscale correlative imaging of the brain Revealing interaction of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM in cosmetic clinical research Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models Bedside assessment of multiphoton tomography
| Author | : Karsten König |
| Publisher | : |
| Total Pages | : 400 |
| Release | : 2016 |
| Genre | : |
| ISBN | : 9783110429992 |
Download Multiphoton Microscopy and Fluorescence Lifetime Imaging Book in PDF, Epub and Kindle
| Author | : Juan Carlos Stockert |
| Publisher | : Bentham Science Publishers |
| Total Pages | : 751 |
| Release | : 2017-12-15 |
| Genre | : Science |
| ISBN | : 1681085186 |
Download Fluorescence Microscopy in Life Sciences Book in PDF, Epub and Kindle
Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.
| Author | : Arthur W. Toga |
| Publisher | : Academic Press |
| Total Pages | : 900 |
| Release | : 2002-10-06 |
| Genre | : Medical |
| ISBN | : 0080528287 |
Download Brain Mapping: The Methods Book in PDF, Epub and Kindle
Investigation of the functional architecture of the human brain using modern noninvasive imaging techniques is a rapidly expanding area of research. A proper knowledge of methodology is needed to appreciate the burgeoning literature in the field. This timely publication provides an excellent catalogue of the main techniques. The authors offer an invaluable analysis of mapping strategies and techniques, providing everything from the foundations to the major pitfalls and practical applications of the modern techniques used in neuroimaging. Contains over 1000 full color pages with more than 200 color figures. Spanning the methodological gamut from the molecular level to the whole brain while discussing anatomy, physiology, and pathology, as well as their integration, Brain Mapping: The Methods, Second Edition, brings the reader a comprehensive, well-illustrated and entirely readable description of the methods for brain mapping. Drs. Toga and Mazziotta provide everything from the foundations to the major pitfalls and practical applications of the technique by assembling an impressive group of experts, all widely known in their field, who contribute an outstanding set of chapters.
| Author | : J. Slavík |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 292 |
| Release | : 2013-11-11 |
| Genre | : Science |
| ISBN | : 1489918663 |
Download Fluorescence Microscopy and Fluorescent Probes Book in PDF, Epub and Kindle
Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.
| Author | : M. H. F. Wilkinson |
| Publisher | : John Wiley & Sons |
| Total Pages | : 588 |
| Release | : 1998-06-08 |
| Genre | : Science |
| ISBN | : 9780471974406 |
Download Digital Image Analysis of Microbes Book in PDF, Epub and Kindle
Providing specific knowledge in the theory of image analysis, optics, fluorescence, and imaging devices in biomedical laboratories, this timely and indispensable volume focuses on the theory and applications of detection, morphometry, and motility measurement techniques applied to bacteria, fungi, yeasts and protozoa.
| Author | : Joseph R. Lakowicz |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 961 |
| Release | : 2007-12-05 |
| Genre | : Science |
| ISBN | : 0387463127 |
Download Principles of Fluorescence Spectroscopy Book in PDF, Epub and Kindle
The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.
