Bioaugmentation for the Remediation of Pesticide-contaminated Soil with Microorganisms Directly Enriched in Soil Or Compost
Author | : Sang-Jun Kim |
Publisher | : |
Total Pages | : |
Release | : 2003 |
Genre | : Bioremediation |
ISBN | : |
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Abstract: EPTC (s-ethyl-N, N'-dipropylthiocarbamate) and atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine) are commonly used herbicides. Atrazine, especially, can be found in soils and waters at unacceptable concentrations. Developing an effective inoculum to bioaugment degradation of EPTC and atrazine in contaminated environments is required for bioremediation of such sites. Microorganisms capable of EPTC or atrazine degradation were directly enriched in soils (hereafter called ACTIVATED soils). The most probable number method revealed that the number of EPTC-degraders in a Brookston soil increased by about 3 logs of magnitude after a single treatment with EPTC at a rate of 20 mg/kg soil. In a Wooster soil, microorganisms utilizing atrazine as their sole carbon or nitrogen source increased by 3 logs and 1 log of magnitude, respectively, after three treatments of atrazine at a rate of 4 mg/kg soil. EPTC or atrazine degradation in these ACTIVATED soils was greatly accelerated after the first treatment, and degradation activity was not inhibited at initial concentrations up to 2000 mg EPTC/kg and 400 mg atrazine/kg soil, respectively. Inoculation of EPTC (20 mg/kg)- or atrazine (4 mg/kg)-contaminated soils with ACTIVATED soil, at rates ranging from 0.05 to 5% (w/w), resulted in significantly (p