Autoradiography: Techniques and Application
Author | : Renato Baserga |
Publisher | : |
Total Pages | : 304 |
Release | : 1969 |
Genre | : Science |
ISBN | : |
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Author | : Renato Baserga |
Publisher | : |
Total Pages | : 304 |
Release | : 1969 |
Genre | : Science |
ISBN | : |
Author | : Renato Baserga |
Publisher | : |
Total Pages | : 281 |
Release | : 1969 |
Genre | : |
ISBN | : |
Author | : Brigitte Schultze |
Publisher | : Academic Press |
Total Pages | : 313 |
Release | : 2013-10-22 |
Genre | : Science |
ISBN | : 1483194876 |
Physical Techniques in Biological Research, Second Edition, Volume III, Part B: Autoradiography at the Cellular Level focuses on high resolution autoradiography with tritium. This book reviews the different autoradiographic techniques and difficulties in theoretical principles needed for the interpretation of autoradiograms. The topics include the characteristics of the different types of radiation; autoradiographic resolution; techniques for applying photographic emulsions; and autoradiography of water-soluble substances. The application of different isotopes; general aspects of the use of thymidine; physiological RNA synthesis; and RNA synthesis under pathological conditions are also elaborated. This text likewise covers the protein synthesis under normal conditions; electron microscopic autoradiography; application of electron microscopic autoradiography; and autoradiographic methods in cytochemistry. This publication is a good reference for students, researchers, and clinicians conducting work on the use of tritiated compounds in high resolution autoradiography.
Author | : P Gahan |
Publisher | : Elsevier |
Total Pages | : 137 |
Release | : 2012-12-02 |
Genre | : Technology & Engineering |
ISBN | : 0323161057 |
Autoradiography for Biologists discusses the basic and practical aspects of various autoradiography techniques. It is composed of six chapters; each chapter covers a specific form of autoradiography technique. Chapter 1 discusses various theoretical aspects of autoradiography, such as radioactive decay, beta-particle absorption, photographic process, sensitivity, and resolving power. Chapters 2 to 6 discuss basic aspects of several forms of autoradiography, including macro-, stripping film, diffusible substances, liquid emulsion, and high resolution autoradiography. Practical and theoretical details of the technique, together with modifications made, are covered in each chapter. This book will be both an encouragement and a help to people wanting to apply autoradiography to the solution of biological problems.
Author | : George A. Boyd |
Publisher | : Academic Press |
Total Pages | : 416 |
Release | : 2013-10-22 |
Genre | : Science |
ISBN | : 1483282333 |
Autoradiography in Biology and Medicine focuses on the applications of autoradiography in medicine and biology, including photographic processes, emulsions, and response of films to isotopes and x-rays. The book first offers information on the meaning of autoradiography, including the etymology of autoradiography, photographic emulsion as a scientific instrument, and the relationship of biologists and autoradiography. The publication then examines the photographic process and comparative response of commercial and scientific emulsions. Discussions focus on fixation, washing, emulsion, response of films to electrons, x-rays, and gamma rays, and response of films to beta ray spectra of isotopes. The text takes a look at commercial photographic materials for autoradiography, autoradiographic image, and resolution. The manuscript then reviews the estimation of dose and exposure time, sources of error, and techniques. Topics include chemical variables, estimation of exposure time, errors occurring during the preparation of the autoradiogram for exposure, and contour autoradiography. The book is a valuable reference for readers interested in autoradiography.
Author | : M.A. Williams |
Publisher | : Elsevier |
Total Pages | : 238 |
Release | : 2013-10-22 |
Genre | : Science |
ISBN | : 1483293874 |
Autoradiography and Immunocytochemistry sets out in detail the preparation of autoradiographs and methods of preparing and use of immunological staining reagents. This book begins with an introduction to the demand for autoradiography and immunocytochemistry and their most advantageous use, followed by a discussion on the immunocytochemistry at the electron microscopical (EM) level in relation to the preparation of labeled antibodies. Other topics include the general remarks on “staining with antibodies; applying Fer-Ab conjugates to ultrathin sections; and controls in immunoferritin staining. The multistep antibody staining methods with unmodified proteins; control observations in staining experiments using lectins; and pattern analysis in EM immunocytochemistry are also covered. This text concludes with a description of the preparation of electron microscope autoradiographs, including the radioisotopes in EM autoradiography, preparation of radioactively-labeled tissues and cells, and methods of applying nuclear emulsions. This publication serves as a detailed laboratory guide for researchers and workers to successfully conduct electron microscope techniques.
Author | : Renato Baserga |
Publisher | : |
Total Pages | : 304 |
Release | : 1969 |
Genre | : Science |
ISBN | : |
Author | : Andrew W. Rogers |
Publisher | : |
Total Pages | : 360 |
Release | : 1969 |
Genre | : Autoradiography |
ISBN | : |
Author | : H. A. Fischer |
Publisher | : Walter de Gruyter GmbH & Co KG |
Total Pages | : 212 |
Release | : 2020-05-18 |
Genre | : Medical |
ISBN | : 3112318498 |
No detailed description available for "Autoradiography".
Author | : Walle J.H. Nauta |
Publisher | : Springer |
Total Pages | : 0 |
Release | : 2012-04-25 |
Genre | : Medical |
ISBN | : 9783642859885 |
As a source of information on neuroanatomical research methods this Volume is not without precedent. In 1957, at the initiative of Dr. W. F. Windle, a conference was held at the National Institutes of Health, the proceedings of which, edited by Dr. Windle and published by C. C. Thomas under the title "New Research Tech niques of Neuroanatomy", rapidly became something like a standard reference in the field of Neuromorphology. The present editors were emboldened to seek support for a second expose of contemporary research methods in Neuroanatomy by the success of this earlier publication, as well as by the consideration that the years elapsed since its appearance have been, perhaps, more productive of new research methods and strategies in Neuroanatomy than were any dozen consecutive years since the golden decades of the 1870's and 1880's. The decision, which methods to include in this conference, has been a difficult one. For reasons of space alone it would have been impossible to do equal justice to techniques approaching the brain as a neuronal system, the brain as a tissue, or the neuron as a cell. As a brief inspection of the contents of this volume will show, the weight of choice fell upon the first of these alternatives. The reader will find, further more, that not all of the book is devoted to new methods in the strict sense.