Analysis Of The Rna And Terminal Sequences Of Vesicular Stomatitis Virus And Its Defective Interfering Particles
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Author | : Bert Lawrence Semler |
Publisher | : |
Total Pages | : 192 |
Release | : 1979 |
Genre | : |
ISBN | : |
Download Analysis of the RNA and Terminal Sequences of Vesicular Stomatitis Virus and Its Defective Interfering Particles Book in PDF, Epub and Kindle
Author | : Woo-Young Choi |
Publisher | : |
Total Pages | : 326 |
Release | : 1997 |
Genre | : Stomatitis |
ISBN | : |
Download Molecular Biological Characterization of Defective Interfering Particles of Vesicular Stomatitis Virus Book in PDF, Epub and Kindle
Author | : Cheryl Isaac Murphy |
Publisher | : |
Total Pages | : 244 |
Release | : 1982 |
Genre | : Proteins |
ISBN | : |
Download Protein/RNA Structural Relationships in the Nucleocapsids of Vesicular Stomatitis Virus and Its Defective Interfering Particles Book in PDF, Epub and Kindle
Author | : Nicholas James De Polo |
Publisher | : |
Total Pages | : 292 |
Release | : 1985 |
Genre | : Viruses |
ISBN | : |
Download Complex Coevolution of Vesicular Stomatitis Virus and Its Defective Interfering Particles Book in PDF, Epub and Kindle
Author | : David Marc Canter |
Publisher | : |
Total Pages | : 320 |
Release | : 1997 |
Genre | : Genetic transcription |
ISBN | : |
Download Roles of P and L Proteins in Regulating Vesicular Stomatitis Virus RNA Synthesis Analyzed by a Reverse Genetic Approach Book in PDF, Epub and Kindle
Nonsegmented, negative strand RNA viruses are medically important in that they represent a broad class of infectious agents (three families - Rhabdoviridae, Paramyxoviridae and Filoviridae), including such afflictions as rabies (Rhabdoviridae) and Ebola virus (Filoviridae). Much of what we know about these viruses comes from studies carried out with vesicular stomatitis virus, the prototypic member of the family rhabdoviridae. VSV encodes a 29-kD phosphoprotein (P) and 241-kD large (L) protein which together form the polymerase complex responsible for transcription and replication. The mechanism regulating the switch between these two distinct modes of RNA synthesis is unknown and is the focus of this dissertation. Previous studies suggested involvement of an ATP-dependent function. Initially, a novel in vitro transcription reconstitution system was developed using plasmid encoded P and L proteins that both faithfully and efficiently mimicked in vitro transcription from disrupted virion cores. L protein was shown to be highly unstable (half-life 3 to 6 hours) when expressed alone and required P protein coexpression for its stability. Using the in vitro transcription reconstitution assay, constitutive phosphorylation of P protein was shown to be non-essential for transcription despite the claims of other labs; however, this modification appears to play a role in P multimerization and polymerase complex formation. L proteins containing mutations in a universally conserved NTP-binding motif were engineered and analyzed by an in vivo transcription/replication assay. All such mutants were shown to stimulate replication over wild-type L protein in a promoter-specific manner while they concomitantly down regulating transcription in a promoter-independent manner. Glycerol gradient analysis revealed that wild-type L protein, but not mutant L, displayed a higher sedimentation rate in the presence of ATP. Taken together, these results suggest that different modes of polymerization are likely dependent on a conformational switch of the L protein and that the ATP-bound form of the polymerase is a transcriptase while the unbound form is a replicase. These findings reveal a new mechanism by which polymerases are regulated and define a new target for antiviral strategies.
Author | : Bishop |
Publisher | : CRC Press |
Total Pages | : 245 |
Release | : 2018-05-04 |
Genre | : Science |
ISBN | : 1351084844 |
Download Rhabdoviruses Book in PDF, Epub and Kindle
The goal of this book series has been to provide an overview of rhabdovirology as a whole (including an appraisal of current research findings), suitable for students, teachers, and, research workers. To realize this goal many of the research leaders in the different disciplines of rhabdovirology were asked to contribute chapters.
Author | : Stephan Scott Monroe |
Publisher | : |
Total Pages | : 226 |
Release | : 1983 |
Genre | : RNA. |
ISBN | : |
Download Sequence Analysis of the RNA Isolated from Defective Interfering Particles of Sindbis Virus Book in PDF, Epub and Kindle
Author | : Jacques Perrault |
Publisher | : |
Total Pages | : 262 |
Release | : 1972 |
Genre | : |
ISBN | : |
Download Homologous Auto-interference in Vesicular Stomatitis Virus Book in PDF, Epub and Kindle
Author | : |
Publisher | : |
Total Pages | : 0 |
Release | : 2013 |
Genre | : |
ISBN | : |
Download Kinetics of Vesicular Stomatitis Virus MRNA and Genomes During Infection Book in PDF, Epub and Kindle
Viruses can be attributed to many devastating human diseases. In this thesis I present quantitative methods and analysis of infections of vesicular stomatitis virus (VSV) in cell culture environments. The work presented here reveals new information about VSV infection and suggests future directions for quantitative study of viral infections. Quantitative data collection and analysis in Chapter 2 reveal that the mRNA production rate is dependent only on viral genomes, suggesting that viral polymerases are in excess. This work suggests that VSV is able to establish a robust infection in multiple cellular environments. In Chapter 3 I present a large scale simulation and simulation environment developed to predict the outcome of infections of multiple strains of VSV in cell culture. The simulation reveals that viral genome replication is limited by the presence of the nucleocapsid protein, the catalyst for genome replication. Finally, in Chapter 4 I present quantitative analysis of VSV populations using RNA sequencing. This work shows that RNA sequencing is an unbiased tool that can be used to detect, identify, and quantify defective interfering particles in a virus population. Together this work shows that quantitative analysis of biological systems can reveal new biology. Quantitative techniques are necessary for developing predictive models of viral infections, which will lead to the rational design of anti-viral treatments.
Author | : |
Publisher | : |
Total Pages | : |
Release | : 1973 |
Genre | : |
ISBN | : |
Download The Relationships of Some Defective Interfering Particles to The Infectious Virion of Vesicular Stomatitis Virus by Analysis of The Annealing Properties of Their Ribonucleic Acid Book in PDF, Epub and Kindle