Biomedicine and bioinformatics engineering are interdisciplinary fields combining expertise from biology, mathematics, chemistry, computer science, and engineering to develop technologies which will address major problems at the forefront of biomedical and bio-industrial research. This book presents the proceedings of ICBBE 2023, the 3rd International Conference on Biomedicine and Bioinformatics Engineering, held as a hybrid event from 16-18 June 2023 in Nanjing, China. The aim of the conference was to create a forum for the multi-disciplinary discussion of recent developments in biomedicine and bioinformatics engineering. A total of 253 submissions were received for the conference, of which 92 were accepted after a thorough double-blind peer review. The book is divided into 3 parts, covering biomedical material and imaging technology application; cell biology and medical signal processing; and biomechanical modeling and drug analysis, and topics addressed include biomedical signal processing; medical information; bioinformatics and computational biology; medical imaging technology and its application; molecular biology; chemistry, pharmacology and toxicology. Addressing a number of highly relevant aspects of biomedicine and bioinformatics engineering and emphasizing the multi-disciplinary aspects of the field, the selected contributions in this book will provide valuable guidance for future interdisciplinary developments, and will be of interest to all those working in biomedicine and bioinformatics engineering.