Theory, Techniques and Applications of Nanotechnology in Gene Silencing

Theory, Techniques and Applications of Nanotechnology in Gene Silencing
Author: Surendra Nimesh
Publisher: CRC Press
Total Pages: 139
Release: 2022-09-01
Genre: Science
ISBN: 1000819280

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This book focuses on various aspects of nanotechnology based gene silencing. The initial chapters detail the techniques available for in vitro and in vivo characterization of nanoparticles. In the later chapters, exhaustive details about various polymeric systems employed for gene silencing has been accounted.

Gene Silencing

Gene Silencing
Author: Anthony J. Catalano
Publisher: Nova Biomedical Books
Total Pages: 361
Release: 2011
Genre: Medical
ISBN: 9781617286056

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Advances in Nanomedicine for the Delivery of Therapeutic Nucleic Acids

Advances in Nanomedicine for the Delivery of Therapeutic Nucleic Acids
Author: Surendra Nimesh
Publisher: Woodhead Publishing
Total Pages: 258
Release: 2017-04-04
Genre: Medical
ISBN: 0081005636

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Advances in Nanomedicine for the Delivery of Therapeutic Nucleic Acids addresses several issues related to safe and effective delivery of nucleic acids (NAs) using nanoparticles. A further emphasis would be laid on the mechanism of delivery of NAs, the barriers encountered and the strategies adapted to combat them. An exhaustive account of the advantages as well shortcomings of all the delivery vectors being employed in delivery of various NAs will be provided. On final note the regulatory aspects of nanoparticles mediated NA would be discussed, with focus on their clinical relevance. The design and development of nucleic acid-based therapeutics for the treatment of diseases arising from genetic abnormalities has made significant progress over the past few years. NAs have been widely explored for the treatment of cancer and infectious diseases or to block cell proliferation and thereby caused diseases. Advances in synthetic oligonucleotide chemistry resulted in synthesis of NAs that are relatively stable in in vivo environments. However, cellular targeting and intracellular delivery of NAs still remains a challenge. Further development of NA-based therapeutics depends on the progress of safe and effective carriers for systemic administration. Nanomedicine has facilitated availability of vectors with diminished cytotoxicity and enhanced efficacy which are rapidly emerging as systems of choice. These vectors protect NAs from enzymatic degradation by forming condensed complexes along with targeted tissue and cellular delivery. During the past few years, a myriad reports have appeared reporting delivery of NAs mediated by nanoparticles. This book will provide an overview of nanoparticles being employed in the in vitro and in vivo delivery of therapeutically relevant NAs like DNA, siRNA, LNA, PNA, etc. Provides a complete overview of the applicatiosn of nanomedicine in the delivery of nucleic acids, from characterization of nanoparticles, to in vitro and in vivo studies Discusses delivery issues of less well explored nucleic acids, like PNAs, Ribozymes, DNAzymes, etc. Summarizes the current state of research in nucleic acid delivery and underscores the future of nanomedicine in this field

Nanomaterial Characterization

Nanomaterial Characterization
Author: Ratna Tantra
Publisher: John Wiley & Sons
Total Pages: 320
Release: 2016-03-24
Genre: Science
ISBN: 1118753933

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Nanomaterial Characterization Providing various properties of nanomaterials and the various methods available for their characterization Over the course of the last few decades, research activity on nanomaterials has gained considerable press coverage. The use of nanomaterials has meant that consumer products can be made lighter, stronger, esthetically more pleasing, and less expensive. The significant role of nanomaterials in improving the quality of life is clear, resulting in faster computers, cleaner energy production, target-driven pharmaceuticals, and better construction materials. It is not surprising, therefore, that nanomaterial research has really taken off, spanning across different scientific disciplines from material science to nanotoxicology. A critical part of any nanomaterial research, however, is the need to characterize physicochemical properties of the nanomaterials, which is not a trivial matter. Nanomaterial Characterization: An Introduction is dedicated to understanding the key physicochemical properties and their characterization methods. Each chapter begins by giving an overview of the topic before a case study is presented. The purpose of the case study is to demonstrate how the reader may make use of the background information presented to them and show how this can be translated to solve a nanospecific application scenario. Thus, it will be useful for researchers in helping them design experimental investigations. The book begins with a general overview of the subject, thus giving the reader a solid foundation to nanomaterial characterization. Nanomaterial Characterization: An Introduction features: Nanomaterial synthesis and reference nananomaterials Key physicochemical properties and their measurements including particle size distribution by number, solubility, surface area, surface chemistry, mechanical/tribological properties, and dustiness Scanning tunneling microscopy methods operated under extreme conditions Novel strategy for biological characterization of nanomaterial methods Methods to handle and visualize multidimensional nanomaterial characterization data The book is written in such a way that both students and experts in other fields of science will find the information useful, whether they are in academia, industry, or regulation, or those whose analytical background may be limited.There is also an extensive list of references associated with every chapter to encourage further reading.

Selected Topics In Nanomedicine

Selected Topics In Nanomedicine
Author: Thomas Ming Swi Chang
Publisher: World Scientific
Total Pages: 599
Release: 2013-09-16
Genre: Medical
ISBN: 9814472875

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Nanomedicine consists of the use of nanotechnology and nanobiotechnology in medicine. There have been extensive developments in the area of nanomedicine. The scope of this book is first to discuss the origin of nanomedicine. Following this, instead of a general overview of the whole area, 24 chapters on selected topics of important areas are described in detail. Authors are selected from around the world to give a representative and international view of the activities in the area of nanomedicine.

Gene therapy

Gene therapy
Author: Surendra Nimesh
Publisher: Elsevier
Total Pages: 380
Release: 2013-10-31
Genre: Science
ISBN: 1908818646

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Gene therapy is emerging as a new class of therapeutics for the treatment of inherited and acquired diseases. However, poor cellular uptake and instability of DNA in the physiological milieu limits its therapeutic potential, hence a vector which can protect and efficiently transport DNA to the target cells must be developed. Nanotechnology-based non-viral vectors have been proposed as potential candidates. Various polymeric nanoparticles have been shown to be suitable, with high cellular uptake efficiencies and reduced cytotoxicity. These delivery vectors form condensed complexes with DNA which result in shielding against enzymatic degradation and enhanced cellular targeting. Advantages including easy manipulatibility, high stability, low cost and high payload, mean that nanoparticles from various polymers have been exploited. Gene therapy gives a systematic account of the many aspects of nanotechnology mediated gene therapy, from the preparation of nanoparticles to physicochemical characterization, and follows with applications in in vitro and in vivo models. This book emphasizes the various aspects of nanotechnology-based gene therapy, with initial chapters detailing the tools and techniques available for preparation and in vitro and in vivo characterization of nanoparticles. Later chapters provide exhaustive details on polymeric systems employed for gene therapy. Provides an overview of nanotechnology applications in gene therapy, from preparation of nanoparticles to in vitro and in vivo studies Details the tools and techniques available for preparation, characterization and in vitro and in vivo study of nanoparticles Details the limitations of nanoparticle-mediated gene therapy and proposes ways in which they may be overcome

Handbook of Research on Nanoscience, Nanotechnology, and Advanced Materials

Handbook of Research on Nanoscience, Nanotechnology, and Advanced Materials
Author: Bououdina, Mohamed
Publisher: IGI Global
Total Pages: 641
Release: 2014-03-31
Genre: Technology & Engineering
ISBN: 1466658258

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The burgeoning field of nanotechnology has led to many recent technological innovations and discoveries. Understanding the impact of these technologies on business, science, and industry is an important first step in developing applications for a variety of settings and contexts. Handbook of Research on Nanoscience, Nanotechnology, and Advanced Materials presents a detailed analysis of current experimental and theoretical approaches surrounding nanomaterials science. With applications in fields such as biomedicine, renewable energy, and synthetic materials, the research in this book will provide experimentalists, professionals, students, and academics with an in-depth understanding of nanoscience and its impact on modern technology.

Oligonucleotide Conjugates in DNA Nanotechnology

Oligonucleotide Conjugates in DNA Nanotechnology
Author: Alexander Prinzen
Publisher:
Total Pages:
Release: 2022
Genre:
ISBN:

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"Oligonucleotides, in the form of DNA and RNA are primarily known as the information and messenger molecules of the cell. Roles beyond this informational molecule have seen oligonucleotides used as structural materials, therapeutics, and molecular recognition components. Combining many of these different roles, the field of DNA nanotechnology has emerged, to generate an unprecedented number of different devices, for applications in sensing, diagnostics, gene silencing and drug delivery. Especially in the case of gene silencing and drug delivery, the biocompatibility, and recognition abilities of DNA place these nanostructures as advantageous carriers for the transport and selective release of these agents. However, by its nature, DNA is chemically inert and to gain more function, chemical modifications are generally needed. This thesis presents new oligonucleotide conjugates used in combination with DNA nanotechnology for the selective delivery/release of both small molecule drugs and antisense oligonucleotides. At first, the evolution of a DNA device that was engineered to selectively amplify the release of small molecule therapeutics, in response to a molecular recognition event is described. A detailed investigation into the design, conjugation chemistry, and kinetics of release are presented. Furthermore, integrating structural DNA nanotechnology and the drug release platform together allows for the generation of well-defined nanostructures which can act as standalone devices without multiple components. Secondly, a new antisense oligonucleotide conjugated with hydrophobic disulfide modifications is generated for their ability to self-assemble into spherical nucleic acids and produce stimuli responsive gene silencing under reducing conditions. The stability of these structures in cellular media and uptake into cells of these structures is thoroughly investigated. Finally, different strategies to improve the drug release device from Chapter 2 for transitioning it into real biological drug delivery applications is developed. Factors such as off-target degradation/activation, delivery to the site of action, rate of metabolism and overall drug specificity are addressed"--

Handbook of Nanomaterials for Cancer Theranostics

Handbook of Nanomaterials for Cancer Theranostics
Author: Joao Conde
Publisher: Elsevier
Total Pages: 608
Release: 2018-06-27
Genre: Science
ISBN: 0128133406

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Handbook of Nanomaterials for Cancer Theranostics focuses on recent developments in advanced theranostic nanomedicines from a chemical and biological perspective where the advantages of theranostics are achieved by combining multiple components. The authors explore the pros and cons of theranostic nanomaterials developed in cancer research in the last 15 years, with the different strategies compared and scrutinized. In addition, the book explores how nanomaterials may overcome the regulatory hurdles facing theranostic nanomedicines. This is an important research reference for postgraduates and researchers in nanomedicine and cancer research who want to learn more on how nanomaterials can help create more effective cancer treatments. Highlights the development of smart theranostic nanomaterials to tackle biomedical problems in cancer therapy and diagnostics Explores the regulatory hurdles facing theranostic nanomedicine Discusses how the use of nanomaterials can help create more effective cancer treatments