Spectroscopy for the Biological Sciences

Spectroscopy for the Biological Sciences
Author: Gordon G. Hammes
Publisher: John Wiley & Sons
Total Pages: 192
Release: 2005-08-19
Genre: Science
ISBN: 0471733547

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An introduction to the physical principles of spectroscopy and their applications to the biological sciences Advances in such fields as proteomics and genomics place new demands on students and professionals to be able to apply quantitative concepts to the biological phenomena that they are studying. Spectroscopy for the Biological Sciences provides students and professionals with a working knowledge of the physical chemical aspects of spectroscopy, along with their applications to important biological problems. Designed as a companion to Professor Hammes's Thermodynamics and Kinetics for the Biological Sciences, this approachable yet thorough text covers the basic principles of spectroscopy, including: * Fundamentals of spectroscopy * Electronic spectra * Circular dichroism and optical rotary dispersion * Vibration in macromolecules (IR, Raman, etc.) * Magnetic resonance * X-ray crystallography * Mass spectrometry With a minimum of mathematics and a strong focus on applications to biology, this book will prepare current and future professionals to better understand the quantitative interpretation of biological phenomena and to utilize these tools in their work.

Vibrational Spectroscopy in Life Science

Vibrational Spectroscopy in Life Science
Author: Friedrich Siebert
Publisher: John Wiley & Sons
Total Pages: 320
Release: 2008-07-15
Genre: Science
ISBN: 3527621350

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The authors describe basic theoretical concepts of vibrational spectroscopy, address instrumental aspects and experimental procedures, and discuss experimental and theoretical methods for interpreting vibrational spectra. It is shown how vibrational spectroscopy provides information on general aspects of proteins, such as structure, dynamics, and protein folding. In addition, the authors use selected examples to demonstrate the application of Raman and IR spectroscopy to specific biological systems, such as metalloproteins, and photoreceptors. Throughout, references to extensive mathematical and physical aspects, involved biochemical features, and aspects of molecular biology are set in boxes for easier reading. Ideal for undergraduate as well as graduate students of biology, biochemistry, chemistry, and physics looking for a compact introduction to this field.

Spectroscopy in Biology and Chemistry

Spectroscopy in Biology and Chemistry
Author: Sow-Hsin Chen
Publisher:
Total Pages: 434
Release: 1974
Genre: Science
ISBN:

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Spectroscopy in Biology and Chemistry discusses the use of thermal neutron diffraction and inelastic scattering, and the related techniques of x-ray diffraction, Raman and Rayleigh scattering, in investigating biological macromolecules and chemical systems. The book describes neutron, x-ray and laser spectroscopy; quasielastic scattering in neutron and laser spectroscopy; and interatomic forces, molecular structure and molecular vibrations. The text also discusses the x-ray crystallography of biological molecules; neutron diffraction studies of hydrogen bonding in organic and biochemical syste ...

Applications of Raman Spectroscopy to Biology

Applications of Raman Spectroscopy to Biology
Author: M. Ghomi
Publisher: IOS Press
Total Pages: 204
Release: 2012-02-23
Genre: Medical
ISBN: 1614990204

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Raman spectroscopy has been known and used as a technique for 80 years, originally for the study of inorganic substances. Recent advances in underlying technology, such as lasers, detectors, filters and components, have transformed the technique into a very effective modern tool for studying complex biological problems. Professor Mahmoud Ghomi (of the University of Paris XIII) has edited this book on the applications of Raman spectroscopy to biology, covering in a readily accessible way the area from basic studies to the diagnosis of disease. The early chapters provide background information on basic principles underlying the main Raman methods covered in the book, with information on Surface-Enhanced Raman Scattering (SERS) and Surface-Enhanced Fluorescence (SEF), as well as giving accounts of applications to biomolecular and cellular investigations. Among the topics covered are studies of drugs and their complexes with biomolecules on nanoparticles, application of SERS to blood analysis, studies of single cells and of applications to human cancer diagnostics.This will be a useful book for experimental scientists in academic, governmental, industrial and clinical environments and for those entering the field of biomolecular spectroscopy.

In-cell NMR Spectroscopy

In-cell NMR Spectroscopy
Author: Yutaka Ito
Publisher: Royal Society of Chemistry
Total Pages: 322
Release: 2019-12-13
Genre: Science
ISBN: 1788012178

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In-cell NMR spectroscopy is a relatively new field. Despite its short history, recent in-cell NMR-related publications in major journals indicate that this method is receiving significant general attention. This book provides the first informative work specifically focused on in-cell NMR. It details the historical background of in-cell NMR, host cells for in-cell NMR studies, methods for in-cell biological techniques and NMR spectroscopy, applications, and future perspectives. Researchers in biochemistry, biophysics, molecular biology, cell biology, structural biology as well as NMR analysts interested in biological applications will all find this book valuable reading.

Biological and Biomedical Infrared Spectroscopy

Biological and Biomedical Infrared Spectroscopy
Author: Andreas Barth
Publisher: IOS Press
Total Pages: 448
Release: 2009
Genre: Science
ISBN: 1607500450

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Although infrared spectroscopy has been applied with success to the study of important biological and biomedical processes for many years, key advances in this vibrant technique have led to its increasing use, ranging from characterization of individual macromolecules (DNA, RNA, lipids, proteins) to human tissues, cells and their components. Infrared spectroscopy thus has a significant role to play in the analysis of the vast number of genes and proteins being identified by the various genomic sequencing projects. Whilst this book gives an overview of the field, it highlights more recent developments, such as the use of bright synchrotron radiation for recording infrared spectra, the development of two-dimensional infrared spectroscopy and the ability to record infrared spectra at ultra fast speeds.

Vibrational Spectroscopy With Neutrons - With Applications In Chemistry, Biology, Materials Science And Catalysis

Vibrational Spectroscopy With Neutrons - With Applications In Chemistry, Biology, Materials Science And Catalysis
Author: Philip C H Mitchell
Publisher: World Scientific
Total Pages: 670
Release: 2005-06-15
Genre: Science
ISBN: 9814482005

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Inelastic neutron scattering (INS) is a spectroscopic technique in which neutrons are used to probe the dynamics of atoms and molecules in solids and liquids. This book is the first, since the late 1960s, to cover the principles and applications of INS as a vibrational-spectroscopic technique. It provides a hands-on account of the use of INS, concentrating on how neutron vibrational spectroscopy can be employed to obtain chemical information on a range of materials that are of interest to chemists, biologists, materials scientists, surface scientists and catalyst researchers. This is an accessible and comprehensive single-volume primary text and reference source.

Fluorescence Spectroscopy in Biology

Fluorescence Spectroscopy in Biology
Author: Martin Hof
Publisher: Springer Science & Business Media
Total Pages: 330
Release: 2004-12-08
Genre: Science
ISBN: 9783540223382

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Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.

Fundamentals of Protein NMR Spectroscopy

Fundamentals of Protein NMR Spectroscopy
Author: Gordon S. Rule
Publisher: Springer Science & Business Media
Total Pages: 543
Release: 2006-02-16
Genre: Science
ISBN: 1402035004

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NMR spectroscopy has proven to be a powerful technique to study the structure and dynamics of biological macromolecules. Fundamentals of Protein NMR Spectroscopy is a comprehensive textbook that guides the reader from a basic understanding of the phenomenological properties of magnetic resonance to the application and interpretation of modern multi-dimensional NMR experiments on 15N/13C-labeled proteins. Beginning with elementary quantum mechanics, a set of practical rules is presented and used to describe many commonly employed multi-dimensional, multi-nuclear NMR pulse sequences. A modular analysis of NMR pulse sequence building blocks also provides a basis for understanding and developing novel pulse programs. This text not only covers topics from chemical shift assignment to protein structure refinement, as well as the analysis of protein dynamics and chemical kinetics, but also provides a practical guide to many aspects of modern spectrometer hardware, sample preparation, experimental set-up, and data processing. End of chapter exercises are included to emphasize important concepts. Fundamentals of Protein NMR Spectroscopy not only offer students a systematic, in-depth, understanding of modern NMR spectroscopy and its application to biomolecular systems, but will also be a useful reference for the experienced investigator.

Spectroscopy of Biological Molecules

Spectroscopy of Biological Molecules
Author: Camille Sandorfy
Publisher: Springer Science & Business Media
Total Pages: 639
Release: 2012-12-06
Genre: Science
ISBN: 9400964900

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This volume contains the proceedings of the NATO-Advanced Study Institute on the "Spectroscopy of Biological Molecules", which took place on July 4-15, 1983 in Acquafredda di Maratea, Italy. The institute concentrated on three main subiects: the structure and dymanics of DNA, proteins, and visual and plant pigments. Its timeliness has been linked to rapid advances in certain spectroscopic techniques which yielded a consider able amount of new information on the structure and inter actions of biologically important molecules. Among these techniques Fourier transform infrared, resonance and surface enhanced 'Raman spectroscopies, Raman microscopy and micro probing, time resolved techniques, two photon and ultrafast electronic, and C-13, N-15 and P-31 NMR spectroscopies and kinetic and static IR difference spectroscopy receiced a great deal of attention at the Institute. In addition, an entirely new technique, near-millimeter-wave spectroscopy has been presented and discussed. Two introductory quantum chemical lectures, one on the structure of water in DNA, and another pn the energy bands in DNA and proteins set the stage for the experimentally oriented lectures that followed. Fundamental knowledge on hydrogen bonding was the topic of two other lectures. Panel discussions were held on the structure and confor mations of DNA, metal-DNA adducts and proteins and on visual pigments. Many scientists who normally attend different conferences and never meet, met at Aquafredda di Maratea. We feel, that at the end of our Institute a synthetic vi~w emerged on the powerful spectroscopic and theoretical methods which are now available for the study of biological molecules.