Physical Chemical Techniques

Physical Chemical Techniques
Author: Dan H. Moore
Publisher: Academic Press
Total Pages: 345
Release: 2013-10-22
Genre: Science
ISBN: 1483194884

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Physical Techniques in Biological Research, Second Edition, Volume II, Part B: Physical Chemical Techniques covers physical chemical techniques that have been most widely employed in the investigation of molecules of biological significance. The book discusses the measurement and properties of ionizing radiations; magnetic resonance and related techniques in magnetic susceptibility measurements; and chromatographic methods and applications of chromatography to steroid research. The text then describes the phenomenon of centrifugation, including the techniques for studying density gradient centrifugation; the formation of gradient column in swinging-bucket tubes; gradient materials; density gradient centrifugation in heavy salt; some factors influencing the distribution of sedimenting particles; and the recovery of samples. Biological investigators and students of biophysics or biochemistry will find the book invaluable.

Cells and Tissues

Cells and Tissues
Author: Gerald Oster
Publisher: Academic Press
Total Pages: 745
Release: 2013-09-03
Genre: Science
ISBN: 1483194922

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Physical Techniques in Biological Research, Volume III: Cells and Tissues focuses on physical techniques applied to the study of cells, determining the morphology, chemical characteristics, and functions of the cellular organelles. This book discusses the microtomy and postdrying treatment of tissues, phase contrast and interference microscopy in cytology, and fluorescence microscopy. The electron microscopy of microorganisms, ultrastructure of layered lipoprotein structures, and techniques for the mass isolation of cellular components are also elaborated. This publication likewise covers the microphotometry with visible light, ultraviolet absorption techniques, and stereoscopic techniques in X-ray microscopy.Other topics include the birefringence and dichroism of cells and tissues, autoradiography at the cellular level, and manometric techniques for single cells. This volume is a good source for biologists and specialists concerned with the study of cells and tissues.