Methodologies for Metabolomics

Methodologies for Metabolomics
Author: Norbert W. Lutz
Publisher: Cambridge University Press
Total Pages: 641
Release: 2013-01-21
Genre: Medical
ISBN: 0521765900
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Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.

Metabolomics

Metabolomics
Author: Wolfram Weckwerth
Publisher: Springer Science & Business Media
Total Pages: 290
Release: 2008-02-04
Genre: Science
ISBN: 1597452440
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Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.

Metabolomics: From Fundamentals to Clinical Applications

Metabolomics: From Fundamentals to Clinical Applications
Author: Alessandra Sussulini
Publisher: Springer
Total Pages: 353
Release: 2017-01-28
Genre: Science
ISBN: 3319476564
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This book provides a comprehensive view of metabolomics, from the basic concepts, through sample preparation and analytical methodologies, to data interpretation and applications in medicine. It is the first volume to cover metabolomics clinical applications while also emphasizing analytical and statistical features. Moreover, future trends and perspectives in clinical metabolomics are also presented. For researches already experienced in metabolomics, the book will be useful as an updated definitive reference. For beginners in the field and graduate students, the book will provide detailed information about concepts and experimental aspects in metabolomics, as well as examples and perspectives of applications of this strategy to clinical questions.

Epigenetics and Metabolomics

Epigenetics and Metabolomics
Author: Paban K. Agrawala
Publisher: Elsevier
Total Pages: 478
Release: 2021-08-25
Genre: Medical
ISBN: 0323856535
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Epigenetics and Metabolomics, a new volume in the Translational Epigenetics series, offers a synthesized discussion of epigenetic control of metabolic activity, and systems-based approaches for better understanding these mechanisms. Over a dozen chapter authors provide an overview of epigenetics in translational medicine and metabolomics techniques, followed by analyses of epigenetic and metabolomic linkage mechanisms likely to result in effective identification of disease biomarkers, as well as new therapies targeting the removal of the inappropriate epigenetic alterations. Epigenetic interventions in cancer, brain damage, and neuroendocrine disease, among other disorders, are discussed in-depth, with an emphasis on exploring next steps for clinical translation and personalized healthcare. Offers a synthesized discussion of epigenetic regulation of metabolic activity and systems-based approaches to power new research Discusses epigenetic control of metabolic pathways and possible therapeutic targets for cancer, neurodegenerative, and neuroendocrine diseases, among others Provides guidance in epigenomics and metabolomic research methodology

Plant Metabolomics

Plant Metabolomics
Author: Carla António
Publisher: Humana
Total Pages: 355
Release: 2019-05-29
Genre: Science
ISBN: 9781493992942
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This detailed book aims to address the need for standardizing key steps in plant metabolomics research that is still considered a critical issue in the field. The collection unites diverse mass spectrometry (MS)-based protocols, from sample preparation and extraction of plant primary and secondary metabolites (e.g. a range of sugars, lipids, phytohormones and volatile organic compounds) to metabolite analysis, using exclusively highly sensitive MS-based methodologies, followed by frontline bioinformatics and/or mathematical modeling approaches to produce reliable biological data interpretation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Metabolomics: Methods and Protocols serves both new and well-established researchers in the plant metabolomics field in finding helpful guidelines and useful laboratory-based protocols for setting up their routine plant metabolomics experiments.

Chromatographic Methods in Metabolomics

Chromatographic Methods in Metabolomics
Author: Tuulia Hyotylainen
Publisher: Royal Society of Chemistry
Total Pages: 259
Release: 2013-09-06
Genre: Science
ISBN: 1849737274
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The concept of a metabolic profile was introduced in 1971, when gas chromatography demonstrated a range of compounds present in human samples. Now termed metabolomics, the field is still emerging, and chromatography remains an essential tool for determining metabolites in a living system. This is the first book to present the chromatographic techniques used in metabolomics in a fundamental way. Sample preparation and quality control are described in detail, and all forms of chromatography applied to metabolomics are included. The editors present guidelines on selecting the most appropriate methodology, making the book an accessible guide to anyone entering the field. Handling data and applications are also described. This is an essential handbook for any laboratory looking to embark on a metabolomics research programme and includes the fundamentals of chromatography alongside the latest developments in the field.

Clinical Metabolomics

Clinical Metabolomics
Author: Martin Giera
Publisher:
Total Pages: 389
Release: 2018
Genre: Biochemistry
ISBN: 9781493975921
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This detailed volume presents a comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations. The methodologies explored here form the core of several very promising initiatives evolving around personalized health care and precision medicine, which can be seen as complimentary to the field of clinical chemistry and aid the aforementioned field with novel disease markers and diagnostic patterns. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.uthoritative and practical, Clinical Metabolomics: Methods and Protocols aims to serve as the basis for successful communication between scientists from several fields, including chemists, biologist, bioinformaticians, and clinicians, ultimately leading to effective study design and completion.

Metabolic Profiling

Metabolic Profiling
Author: Georgios A. Theodoridis
Publisher: Humana
Total Pages: 291
Release: 2019-04-30
Genre: Science
ISBN: 9781493992522
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This volume explores the different approaches and techniques used by researchers to study the recent challenges and developments in metabolic profiling. This book is divided into IV parts. Part I contains chapters that highlight basic concepts, such as experimental design, data treatment, metabolite identification, and harmonization. Part II describes experimental protocols for both targeted and untargeted metabolomics covering the basic analytical technologies: LC-MS, GC-MS, NMR and CE-MS. In addition the protocols describe methods for the study of tissues, feces, blood and other types of biological samples as well as the application of chemical derivatization for GC-MS. Parts III and IV present the use of metabolomics in the study of food, plants and the life sciences, with examples from the quest for the discovery of disease biomarkers, physical exercise omics and metabolic profiling of food, fruit and wine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Metabolic Profiling: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this rapidly developing field.

Metabolomics

Metabolomics
Author: Xianquan Zhan
Publisher: BoD – Books on Demand
Total Pages: 170
Release: 2021-09-01
Genre: Science
ISBN: 1839690836
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Metabolomics is the methodology and theory to study the metabolome, including targeted approaches based on selected/multiple reaction monitoring (SRM/MRM) and untargeted approaches based on nuclear magnetic resonance (NMR) or mass spectrometry (MS). The metabolome contains all metabolites derived from sugars, lipids, proteins, and nucleic acids in a given biological system, tissue, cell, or body fluid in a metabolic network system. Metabolomic variations directly link to molecular mechanisms of a disease, reliable therapeutic targets, and effective biomarkers for prediction, diagnosis, and prognostic assessment of disease. This book presents new advances in the concept and methodology of metabolomics, as well as applications of metabolomics in the research and practice of medical and life sciences.