Magnetic Cell Separation

Magnetic Cell Separation
Author:
Publisher: Elsevier
Total Pages: 473
Release: 2011-08-31
Genre: Science
ISBN: 0080553508

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Cell separation is at the core of current methods in experimental biology and medicine. Its importance is illustrated by the large number of physical and biochemical principles that have been evaluated for application to cell separation. The development of cell separation methods is driven by the needs of biological and medical research, and the ever-increasing demands for sensitivity, selectivity, yield, timeliness and economy of the process. The interdisciplinary nature of research in this area and the volume of information available in research publications and conferences necessitates a basic description of the fundamental processes involved in magnetic cell separation that may help the user in navigating this wealth of information available online and in scientific publications. This book will appeal to researchers in many areas utilizing this technique, including those working in cell biology, clinical research, inorganic chemistry, biochemistry, chemical engineering, materials science, physics and electrical engineering. Provides examples of how to calculate the volume magnetic susceptibility, a fundamental quantity for calculating the magnetic force acting on a cell, from various types of magnetic susceptibilities available in literature Introduces the elements of magnetostatics as they apply to cell magnetization and the magnetization of magnetic micro- and nano- particles used for cell separation Describes the parameters used to determine cell magnetophoresis

Methods of Cell Separation

Methods of Cell Separation
Author: P.T. Sharpe
Publisher: Elsevier
Total Pages: 287
Release: 1988-05-01
Genre: Science
ISBN: 0080858872

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Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; non-specific surface properties; and specific surface properties. The principle of each method is described together with general and, where possible, specific protocols for conducting cell separation experiments. A central theme of the book is the separation of populations of blood lymphocytes which is used as an example for each method. Methods of Cell Separation is distinguished by three powerful assets: descriptions of the majority of cell separation methods currently being used; details of the experimental procedures involved in each method; and comparisons of the different methods for separating cell populations with particular reference to blood lymphocytes. An excellent addition to a distinguished series, and extremely useful as a laboratory manual.

Cell Separation

Cell Separation
Author: Ashok Kumar
Publisher: Springer
Total Pages: 210
Release: 2007-10-09
Genre: Science
ISBN: 3540752633

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With contributions by numerous experts

Molecular Biology of The Cell

Molecular Biology of The Cell
Author: Bruce Alberts
Publisher:
Total Pages: 0
Release: 2002
Genre: Cytology
ISBN: 9780815332183

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Cell Separation Methods and Applications

Cell Separation Methods and Applications
Author: Diether Recktenwald
Publisher: CRC Press
Total Pages: 352
Release: 1997-11-04
Genre: Medical
ISBN: 1482273640

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"Offers complete coverage and assessment of cell separation technologies for analytical and preparative isolations of biological cell populations-demonstrating how to select and devise optimal sorting strategies for applications in biochemistry, immunology, cell and molecular biology, and clinical research. "

Microtechnology for Cell Manipulation and Sorting

Microtechnology for Cell Manipulation and Sorting
Author: Wonhee Lee
Publisher: Springer
Total Pages: 287
Release: 2016-10-05
Genre: Technology & Engineering
ISBN: 3319441396

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This book delves into the recent developments in the microscale and microfluidic technologies that allow manipulation at the single and cell aggregate level. Expert authors review the dominant mechanisms that manipulate and sort biological structures, making this a state-of-the-art overview of conventional cell sorting techniques, the principles of microfluidics, and of microfluidic devices. All chapters highlight the benefits and drawbacks of each technique they discuss, which include magnetic, electrical, optical, acoustic, gravity/sedimentation, inertial, deformability, and aqueous two-phase systems as the dominant mechanisms utilized by microfluidic devices to handle biological samples. Each chapter explains the physics of the mechanism at work, and reviews common geometries and devices to help readers decide the type of style of device required for various applications. This book is appropriate for graduate-level biomedical engineering and analytical chemistry students, as well as engineers and scientists working in the biotechnology industry.

Cell Separation

Cell Separation
Author: Derek Fisher
Publisher: OUP Oxford
Total Pages: 290
Release: 1998-11-26
Genre: Science
ISBN: 0191565555

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Techniques for separating cells are needed in many areas of cell biology. This book presents modern methods from the laboratories of experts in the field, and includes tested, reproducible protocols, hints and tips for success, and troubleshooting suggestions. It will be invaluable to a wide range of cell biologists.

Methods of Cell Separation

Methods of Cell Separation
Author: Nicholas Catsimpoolas
Publisher: Springer Science & Business Media
Total Pages: 211
Release: 2013-03-08
Genre: Medical
ISBN: 1468436627

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Presently, the need for methods involving separation, identification, and characterization of different kinds of cells is amply realized among immu nologists, hematologists, cell biologists, clinical pathologists, and cancer researchers. Unless cells exhibiting different functions and stages of differ entiation are separated from one another, it will be exceedingly difficult to study some of the molecular mechanisms involved in cell recognition, spe cialization, interactions, cytotoxicity, and transformation. Clinical diagno sis of diseased states and use of isolated cells for therapeutic (e. g. , immu notherapy) or survival (e. g, transfusion) purposes are some of the pressing areas where immediate practical benefits can be obtained by applying cell separation techniques. However, the development of such useful methods is still in its infancy. A number of good techniques exist based either on the physical or biological properties of the cells, and these have produced some valuable results. Still others are to be discovered. Therefore, the purpose of this open-ended treatise is to acquaint the reader with some of the basic principles, instrumentation, and procedures presently in practice at various laboratories around the world and to present some typical applications of each technique to particular biological problems.

Cell Separation

Cell Separation
Author: Thomas G. Pretlow
Publisher: Academic Press
Total Pages: 343
Release: 2014-05-10
Genre: Science
ISBN: 1483219380

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Cell Separation: Methods and Selected Applications is a compendium of articles on the design and/or application of methods for the separation of cells. This volume presents contributions on relatively finite subjects on cell separation. It covers topics on cell separation such as methods for obtaining cells in suspension from animal tissues; some of the kinds of data that are helpful in the description of cell purifications; and separation of host cells infiltrating tumors and allografts by velocity sedimentation at unit gravity. The separation of different kinds of nucleated cells from blood by centrifugal elutriation; a new approach to the separation of cells at unit gravity; and the isolation and culture of homogeneous populations of glomerular cell types are elucidated as well. Experimental oncologists, hematologists, immunologists, cell biologists, endocrinologists, and others who are not already expert in the use of methods for cell separation will find the book highly useful.

Cell Separation in Plants

Cell Separation in Plants
Author: Daphne J. Osborne
Publisher: Springer
Total Pages: 449
Release: 2011-11-22
Genre: Science
ISBN: 9783642741630

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This NATO Advanced Research Workshop held 25-30 September, 1988 at the Villa Gualino, Turin, Italy, was the first international meeting of its kind to be devoted solely to cell separation in plants. The partial or complete dissociation of one cell from another is an integral process of differentiation. Partial cell separations are basic physiological components of the overall programme of plant development. Complete cell separations are major events in the ripening of fruits, and the shedding of plant parts. Unscheduled cell separations commonly occur when tissues are subjected to pathogenic invasion. Environmental stresses too, evoke their own separation responses. Over the past five years much new knowledge has been acquired on the regulation of gene expression in specific stages of cell differentiation. Specific molecular markers have been identified that designate the competence of cells for achieving separation. Certain of the chemical signals (hormones, elicitors) that must be emitted or perceived by cells to initiate and sustain separation, are now known to us, and the resulting cell wall changes have come under close chemical scrutiny. The Turin meeting was a focus for those currently involved in such investigations. It assessed factors controlling cell separation in a wide spectrum of different cell types under a variety of conditions.