Autoradiography at the Cellular Level

Autoradiography at the Cellular Level
Author: Brigitte Schultze
Publisher: Academic Press
Total Pages: 313
Release: 2013-10-22
Genre: Science
ISBN: 1483194876

Download Autoradiography at the Cellular Level Book in PDF, Epub and Kindle

Physical Techniques in Biological Research, Second Edition, Volume III, Part B: Autoradiography at the Cellular Level focuses on high resolution autoradiography with tritium. This book reviews the different autoradiographic techniques and difficulties in theoretical principles needed for the interpretation of autoradiograms. The topics include the characteristics of the different types of radiation; autoradiographic resolution; techniques for applying photographic emulsions; and autoradiography of water-soluble substances. The application of different isotopes; general aspects of the use of thymidine; physiological RNA synthesis; and RNA synthesis under pathological conditions are also elaborated. This text likewise covers the protein synthesis under normal conditions; electron microscopic autoradiography; application of electron microscopic autoradiography; and autoradiographic methods in cytochemistry. This publication is a good reference for students, researchers, and clinicians conducting work on the use of tritiated compounds in high resolution autoradiography.

The Use Of Radioautography in Investigating Protein Synthesis

The Use Of Radioautography in Investigating Protein Synthesis
Author: C Leblond
Publisher: Elsevier
Total Pages: 361
Release: 2012-12-02
Genre: Science
ISBN: 0323154328

Download The Use Of Radioautography in Investigating Protein Synthesis Book in PDF, Epub and Kindle

The Use of Radioautography in Investigating Protein Synthesis represents the 1965 Symposium on The Use of Radioautography in Investigating Protein Synthesis, supported by the International Society for Cell Biology. This book is composed of four parts encompassing 18 chapters. The opening part deals first with the basic aspects and mode of operation of the radioautography technique. This part also examines the advantages and potentials of this technique compared to other cytological techniques. The next parts are devoted to the experimental methods of protein synthesis in nucleus and in cells of various types, as revealed by the electron microscope radioautography. The concluding part discusses the secretion and synthesis of protein enzymes by the acinar cells in rat pancreas and fibroblasts in wound healing. This part also considers the application of radioautography to determining the sites of synthesis of proteins. This book is of great value to cell biologists, biochemists, cytologists, histologists, and pathologists.