Area Detectors for Neutron Protein Crystallography

Area Detectors for Neutron Protein Crystallography
Author:
Publisher:
Total Pages: 8
Release: 1992
Genre:
ISBN:

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The main problem in neutron protein crystallography is the low flux of present reactor based or pulsed neutron sources. This low flux is however well matched with presently available multiwire area detectors. One way to increase the flux at the sample is to increase the wavelength bandwidth. The conventional technique uses a monochromator with a bandwidth of the order of 1%. This bandwidth can be increased by using a multilayer monochromator composed of different d'' spacings. This provides a large delta lambda increasing the flux manifold. In this case a reflection in diffraction condition is scanned by the wavelength bandwidth and not by rotation of the reciprocal lattice point through the Ewald sphere. In order to collect most of the simultaneous diffraction a large cylindrical area detector covering an angular width of 120[degrees] with a height of approximately 20cm is needed. Such a detector should have a spacial resolution of 1.1mm, an efficiency of 80% at 2.0A and a counting rate of one million events per second. This detector will be subdivided into 4 sectors that will be decoded simultaneously into a Motorola based computer system. This data acquisition system allows time slicing and this detector is therefore useful for similar experiments using pulsed neutron sources.

Neutron Protein Crystallography

Neutron Protein Crystallography
Author: Nobuo Niimura
Publisher: Oxford University Press
Total Pages: 250
Release: 2011-02-17
Genre: Science
ISBN: 0199578869

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One of the first books dedicated to the emerging field of neutron protein crystallography (NPC). It covers all of the practical aspects of NPC and demonstrates how NPC can explore protein features such as hydrogen bonds, protonation and deprotonation of amino acid residues, and hydration structures.

Data Processing in Neutron Protein Crystallography Using Position-sensitive Detectors

Data Processing in Neutron Protein Crystallography Using Position-sensitive Detectors
Author:
Publisher:
Total Pages:
Release: 1982
Genre:
ISBN:

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Neutrons provide a unique probe for localizing hydrogen atoms and for distinguishing hydrogen from deuterons. Hydrogen atoms largely determine the three-dimensional structure of proteins and are responsible for many catalytic reactions. The study of hydrogen bonding and hydrogen exchange will therefore give insight into reaction mechanisms and conformational fluctuations. In addition, neutrons provide the ability to distinguish N from C and O and to allow correct orientation of groups such as histidine and glutamine. To take advantage of these unique features of neutron crystallography, one needs accurate Fourier maps depicting atomic structure to a high precision. In this paper, techniques are described for minimizing error in the observed structure factors by optimizing data collection and analysis procedures. Special attention is given to subtraction of the high background associated with hydrogen-containing molecules, which produces a disproportionately large statistical error.

Elements of Slow-Neutron Scattering

Elements of Slow-Neutron Scattering
Author: J. M. Carpenter
Publisher: Cambridge University Press
Total Pages: 539
Release: 2015-09-24
Genre: Science
ISBN: 0521857813

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This book provides a comprehensive and up-to-date introduction to the fundamental theory and applications of slow-neutron scattering.

Neutron Scattering in Biology

Neutron Scattering in Biology
Author: Jörg Fitter
Publisher: Springer Science & Business Media
Total Pages: 571
Release: 2006-05-01
Genre: Science
ISBN: 3540291113

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The advent of new neutron facilities and the improvement of existing sources and instruments world wide supply the biological community with many new opportunities in the areas of structural biology and biological physics. The present volume offers a clear description of the various neutron-scattering techniques currently being used to answer biologically relevant questions. Their utility is illustrated through examples by some of the leading researchers in the field of neutron scattering. This volume will be a reference for researchers and a step-by-step guide for young scientists entering the field and the advanced graduate student.

Neutron Scattering

Neutron Scattering
Author:
Publisher: Academic Press
Total Pages: 505
Release: 1987-08-04
Genre: Science
ISBN: 0080860109

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Neutron Scattering

Scattering, Two-Volume Set

Scattering, Two-Volume Set
Author: E. R. Pike
Publisher: Elsevier
Total Pages: 1831
Release: 2001-10-09
Genre: Science
ISBN: 0080540732

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Scattering is the collision of two objects that results in a change of trajectory and energy. For example, in particle physics, such as electrons, photons, or neutrons are "scattered off" of a target specimen, resulting in a different energy and direction. In the field of electromagnetism, scattering is the random diffusion of electromagnetic radiation from air masses is an aid in the long-range sending of radio signals over geographic obstacles such as mountains. This type of scattering, applied to the field of acoustics, is the spreading of sound in many directions due to irregularities in the transmission medium. Volume I of Scattering will be devoted to basic theoretical ideas, approximation methods, numerical techniques and mathematical modeling. Volume II will be concerned with basic experimental techniques, technological practices, and comparisons with relevant theoretical work including seismology, medical applications, meteorological phenomena and astronomy. This reference will be used by researchers and graduate students in physics, applied physics, biophysics, chemical physics, medical physics, acoustics, geosciences, optics, mathematics, and engineering. This is the first encyclopedic-range work on the topic of scattering theory in quantum mechanics, elastodynamics, acoustics, and electromagnetics. It serves as a comprehensive interdisciplinary presentation of scattering and inverse scattering theory and applications in a wide range of scientific fields, with an emphasis, and details, up-to-date developments. Scattering also places an emphasis on the problems that are still in active current research. The first interdisciplinary reference source on scattering to gather all world expertise in this technique Covers the major aspects of scattering in a common language, helping to widening the knowledge of researchers across disciplines The list of editors, associate editors and contributors reads like an international Who's Who in the interdisciplinary field of scattering

Single Crystal Neutron Diffraction from Molecular Materials

Single Crystal Neutron Diffraction from Molecular Materials
Author: Chick C. Wilson
Publisher: World Scientific
Total Pages: 390
Release: 2000
Genre: Science
ISBN: 9789810237769

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This important book presents a comprehensive account of the techniques & applications of single crystal neutron diffraction in the area of chemical crystallography & molecular structure. Beginning with a brief description of the general principles & the reasons for choosing the technique - the "why" - the book covers the methods for both the production of neutrons & the measurement of their scattering by molecular crystals - the "how" - followed by a detailed survey of past, present & future applications - the "what". The coverage of both steady state & pulsed neutron sources & instrumentation is extensive, while the survey of applications is the most comprehensive yet undertaken. The book endeavours to show why the technique is an essential method for studying areas as diverse as hydrogen bonding & weak interactions, organometallics, supramolecular chemistry & crystal engineering, metal hydrides, charge density & pharmaceuticals. It is an ideal reference source for the research worker interested in using neutron diffraction to study the structure of molecules. Contents: Crystallography & the Importance of Structural Information; Neutron Scattering; Neutron Diffractometers; Review of Applications I: The Accurate Location of Atoms; Review of Applications II: Hydrogen Bonding & Other Intermolecular Interactions; Review of Applications III: Probing Vibrations & Disorder; Impact on Material Properties & Design; The Future: New Instruments, New Sources, New Techniques. Readership: Students & researchers involved in structural science, especially chemical crystallography.

Neutron Crystallography in Structural Biology

Neutron Crystallography in Structural Biology
Author:
Publisher: Academic Press
Total Pages: 410
Release: 2020-02-22
Genre: Science
ISBN: 0128192151

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Neutron Crystallography in Structural Biology, Volume 634, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this updated release include Fundamentals of neutron crystallography in structural biology, Large crystal growth for neutron protein crystallography, Prospects for membrane protein crystals in NMX, IMAGINE: The neutron protein crystallography beamline at the high flux isotope reactor, The macromolecular neutron diffractometer at the spallation neutron source, Current status and near future plan of neutron protein crystallography at J-PARC, Neutron macromolecular crystallography at the European spallation source, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on neutron crystallography in structural biology