Analytical Applications of Immobilized Enzyme Reactors

Analytical Applications of Immobilized Enzyme Reactors
Author: S. Lam
Publisher: Springer
Total Pages: 298
Release: 1994
Genre: Medical
ISBN:

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Enzymes are bio-catalysts which effect transformation of substrates to products with high specificity. The usage of enzymes in domestic and industrial applications is well known and has been well documented since the early history of civilization. With the advances in understanding of enzymology, usage of enzymes in industrial and biotechnological pro cesses and molecular medicine has proliferated. One of the key factors in the widespread application of enzymes in modern technologies is the development of enzyme immobilization tech niques, which overcome certain practical, functional and economic con straints. Many natural enzymes can be stabilized by immobilization on solid matrices, with most of the activity retained, for a variety of applica tions. An important application of immobilized enzymes is in liquid chromatography. In the last decade, post-column enzyme detection has become established as an important discipline in liquid chromatography. The new detection approach offers more sensitive and specific ways for measuring major classes of biomolecules. Reactors are fabricated by packing the immobilized enzymes into small columns, which can be placed immediately after an HPLC column.

Analytical Applications of Immobilized Enzymes and Cells

Analytical Applications of Immobilized Enzymes and Cells
Author: Lemuel B. Wingard
Publisher: Elsevier
Total Pages: 329
Release: 2014-06-28
Genre: Technology & Engineering
ISBN: 1483215857

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Analytical Applications of Immobilized Enzymes and Cells, Volume 3 presents a survey of the interdisciplinary interactions, developments, and trends on research in immobilized enzymes and cells. This volume is comprised of eight chapters. Chapters 1 to 4 are devoted to the description of methodologies and instrumentations which are used in routine analysis. Among those described are enzyme tubes, enzyme thermistors, and enzymic or microbial electrodes. Chapter 4 provides a theoretical analysis of electrode design. Subsequent chapters focus on solid-phase enzyme immunoassays and on techniques for in vivo monitoring of metabolites; economic evaluation of the use of high-purity enzymes in analysis; and an assessment of the significance of microprocessors and computer science in enzyme based analysis. Chemists and chemical engineers will find the book very useful.

Analytical Uses of Immobilized Biological Compounds for Detection, Medical and Industrial Uses

Analytical Uses of Immobilized Biological Compounds for Detection, Medical and Industrial Uses
Author: George G. Guilbault
Publisher: Springer Science & Business Media
Total Pages: 398
Release: 2012-12-06
Genre: Science
ISBN: 9400928955

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On May 4-8, 1987, a NATO Advanced Research Workshop on the Analytical Uses of Immobilized Biological Compounds was held in Florence, Italy. The Director of the Workshop was Professor George G. Guilbault of the University of New Orleans, and the Co-Director was Professor Marco Mascini of the University of Florence It vas the purpose of this meeting to assemble scientists from all NATO Countries with an interest in immobilized biological compounds. to discuss - methods of immobilization - properties of immobilized compounds - enzyme electrodes and biosensors - optical devices utilizing immobilized enzymes - microbial sensors and clinical uses of immobilized enzymes - flow injection analysis using enzymes - immobilized biological compounds in chemical defense detection - pharmaceutical analysis - uses in industrial analysis - enzyme reactors - air pollution detectors - immunosensors - medical uses and applications - solid state and FET sensors Goals to be achieved by the conference were - to permit an exchange of views and experience in all these areas - to review and critically assess the state-of-the-art in these fields - to set guidelines for future research and establish collaborative projects between scientists in NATO laboratories in the above areas. Thirty-seven lectures were given by 36 speakers in all of the above areas.

Immobilized Enzymes For Industrial Reactors

Immobilized Enzymes For Industrial Reactors
Author: Ralph Messing
Publisher: Elsevier
Total Pages: 249
Release: 2012-12-02
Genre: Technology & Engineering
ISBN: 0323141323

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Immobilized Enzymes for Industrial Reactors aims to guide the engineer and scientist along the path toward the industrial application of immobilized enzymes. It is necessary to identify the hazards and pitfalls that will be encountered, not only in the initial research efforts, but also during the final engineering phases of a commercial program. Each contributing factor in an immobilized enzyme system will be scrutinized by the authors in an effort to accomplish the overall objectives. This book comprises 10 chapters, with the first being an introduction to and general history of immobilized enzymes. The next chapters go on to discuss basic enzymology; controlled-pore glasses for enzyme immobilization; carriers; immobilization by adsorption and inorganic bridge formation; immobilization by covalent attachment and by entrapment; characteristics of free vs. immobilized enzymes; immobilized coenzymes; design and operation of immobilized enzyme reactors; and applications of immobilized enzymes. This book will be of interest to practitioners in the fields of chemistry and engineering.